山东医药2023，Vol.63Issue(35)：45-50,6.DOI:10.3969/j.issn.1002-266X.2023.35.010
黑果小檗果花色苷提取物对小鼠肝纤维化的改善作用及其机制
Improvement effect and mechanism of extracts from Berberis heteropoda Schrenk total anthocyanins on mice with liver fibrosis
摘要
Abstract
Objective To investigate the improvement effect of extracts from Berberis heteropoda Schrenk total an-thocyanins(BHSTA)on liver fibrosis mice and its mechanism.Methods Forty mice were randomly divided into the nor-mal control group,model control group,low-dose BHSTA group,high-dose BHSTA group,and silybin group,with 8 mice in each group.The hepatic fibrosis models were established by intraperitoneal injection of CCl4 oil in all groups except the normal control group.Mice in the low-dose BHSTA group,high-dose BHSTA group and silybin were given 10 and 40 mg/kg BHSTA solution and 1 μg/10 g silybin by intragastric administration,respectively,while mice in the normal control group and model control group were given equal volume of distilled water by intragastric administration,once a day,for 4 weeks.The body mass of each group was recorded within 4 weeks,and the mice were killed 4 weeks later.The liver index was calculated,and the pathological changes of liver tissues were observed after HE and Masson staining.The serum ala-nine aminotransferase(ALT)and aspartate aminotransferase(AST)were detected by automatic biochemical analyzer.The expression levels of superoxide dismutase(SOD),glutathione peroxidase(GSH)and malondialdehyde(MDA)in liver tis-sues were detected by modified hydroxylamine hydrochloride method.The relative expression levels of NOD-like receptor heat protein domain associated protein 3(NLRP3)and its downstream target gene apoptosis-associated microprotein(ASC),Caspase-1,and interleukin-1β(IL-1β)proteins in the nucleus of liver tissues were detected by Western blotting.Results With the extension of intervention time,the body mass of mice in all groups showed an increasing trend.At the 4th week of group treatment,the body mass of mice in the normal control group,silybin group,high-dose BHSTA group,low-dose BHSTA group and model control group decreased successively,and significant difference was found between ev-ery two groups(all P＜0.05).Compared with normal control group,liver index in the other groups increased,especially in the model control group(all P＜0.05).In the normal control group,the hepatic lobule structure was intact,the hepatic cells were normal,and no inflammatory cell infiltration and collagen fiber hyperplasia were found in the sink area.In the model control group,most of the hepatic sinuses disappeared,moderate or severe edema changes were observed in hepato-cytes,and multiple balloon-like and fatty degeneration were observed,accompanied by multiple inflammatory cell infiltra-tion and a large number of collagen fiber hyperplasia in the portal area.Hepatocyte balloon-like degeneration,inflammato-ry cell infiltration and collagen fiber hyperplasia in the low-dose BHSTA group,high-dose BHSTA group and silybin group were reduced in comparison with those of the model control group.Compared with the normal control group,the expression levels of AST,ALT and MDA in serum and SOD and GSH in liver increased in the model control group,while the expres-sion levels of SOD and GSH in liver decreased(all P＜0.05).Compared with the model control group,the expression lev-els of AST,ALT and MDA in serum decreased in the low-dose BHSTA group,high-dose BHSTA group and silybin group,while the expression levels of SOD and GSH in liver increased.Among them,the decrease in the expression levels of se-rum AST,ALT and MDA in liver tissues was more significant in the high-dose BHSTA group and silybin group,while the increase in the expression levels of SOD and GSH in liver tissues was more significant in the silybin group(all P＜0.05).Compared with the normal control group,the relative expression levels of NLRP3,ASC,Caspase-1 and IL-1β in the liver tissues of the other four groups increased,and the increase was the most significant in the model control group(all P＜0.05).Conclusion BHSTA can protect the liver of mice with hepatic fibrosis,and its mechanism may be related to re-ducing oxidative stress and inhibiting NLRP3 inflammasome-related pathway.关键词
黑果小檗果花色苷提取物/氧化应激/Nod样受体热蛋白结构域相关蛋白3/炎症小体/肝纤维化Key words
extracts from Berberis heteropoda Schrenk total anthocyanins/oxidative stress/Nod-like receptor ther-moprotein domain-associated protein 3/inflammasomes/hepatic fibrosis分类
医药卫生引用本文复制引用
孙吉祥,李倩,刘菁,姚俊英,范旻..黑果小檗果花色苷提取物对小鼠肝纤维化的改善作用及其机制[J].山东医药,2023,63(35):45-50,6.基金项目
新疆维吾尔自治区人民医院院内项目(20190423). （20190423）
