上海针灸杂志2023，Vol.42Issue(12)：1308-1313,6.DOI:10.13460/j.issn.1005-0957.2023.13.3004
电针足三里对失血性休克合并海水浸泡损伤大鼠的作用研究
Study of the effect of electroacupuncture at Zusanli(ST36)on rat models of hemorrhagic shock combined with seawater immersion injury
摘要
Abstract
Objective To study the effects of electroacupuncture(EA)at Zusanli(ST36)on the mean arterial pressure(MAP),heart rate(HR),lactate(LAC),and blood flow in the abdominal organs of rats with delayed intravenous rehydration after the hemorrhagic shock(HS)and seawater immersion injury.Method Eighty-four SPF-grade male Sprague-Dawley rats were selected and divided into an HS+seawater immersion+delayed intravenous rehydration group(control group)and an HS+seawater immersion+EA at ST36+delayed intravenous rehydration group(EA group).Rapid bloodletting through the right common carotid artery was performed till the blood loss reached 45%of the total blood volume,immediately followed by immersion of the body from 0.5 cm below the xiphoid process in the artificial seawater at(23±1)℃for 30 min.Two hours after seawater immersion,the extracted blood and lactated Ringer's solution twice of the volume of the blood loss were intravenously infused.The EA group received EA at bilateral Zusanli 0.5 h after seawater immersion,while the control group was only fixed without interventions.Changes in the MAP,HR,LAC and abdominal organ blood flow were monitored before blood loss(the baseline),immediately after immersion,and 2 h,5 h,and 24 h after immersion.Result Immediately after immersion,the MAP and HR were significantly lower in both groups than before blood loss(P＜0.01),and the MAP and HR were significantly higher in the EA group than in the control group 2 h after immersion(P＜0.05).The LAC level was significantly higher immediately after immersion than before blood loss in both groups(P＜0.01),and the LAC level was markedly lower in the EA group than in the control group 5 h after immersion(P＜0.05).The blood flow volume in the kidney,liver,and intestinal mucosa decreased significantly in both groups after seawater immersion compared with that before blood loss(P＜0.01).The blood flow volume in the kidney,liver,and intestinal mucosa notably improved in the EA group compared with the control group 2 h after immersion(P＜0.01),and the blood flow in the liver and small intestinal mucosa was still notably larger in the EA group than in the control group 5 h after immersion(P＜0.05).Conclusion EA at Zusanli can significantly increase the MAP and HR,reduce blood LAC level,and improve blood flow in the abdominal organs of the animal model of hemorrhagic shock combined with seawater immersion,thus having certain anti-shock effects on sea warfare injuries.关键词
电针/穴,足三里/休克,失血性/海水浸泡/大鼠Key words
Electroacupuncture/Point,Zusanli(ST36)/Shock,Hemorrhagic/Seawater immersion/Rats分类
医药卫生引用本文复制引用
王莉莉,胡森,张海湃,田雨沫,孟祥熙,张慧苹,关玲..电针足三里对失血性休克合并海水浸泡损伤大鼠的作用研究[J].上海针灸杂志,2023,42(12):1308-1313,6.基金项目
国家自然科学基金项目(81774422) （81774422）
军队十三五项目(BHJ17J010) （BHJ17J010）
