上海针灸杂志2023，Vol.42Issue(12)：1314-1324,11.DOI:10.13460/j.issn.1005-0957.2023.13.3005
电针对干眼兔泪腺细胞凋亡及相关蛋白表达的影响
Effects of electroacupuncture on lacrimal gland cell apoptosis and related protein expression in dry eye rabbits
摘要
Abstract
Objective To discuss the regulatory effect of electroacupuncture(EA)on lacrimal gland cell apoptosis in dry eye rabbits.Method Male New Zealand rabbits were randomly divided into a normal group,a model group,an EA group,and a sham EA group.The dry eye rabbit model was prepared via eye exposure to Benzalkonium chloride solution.The normal group received neither modeling nor interventions.The model group only received the same fixing as the EA group after modeling.The EA and sham EA groups received EA or sham EA interventions,respectively,after modeling.Before and after the intervention,the ocular surface function was examined using the Schirmer Ⅰ test(SⅠT),tear break-up time(TBUT),and corneal fluorescein sodium(CFS)score.The terminal deoxynucleotidyl transferase mediated dUTPnick-end labeling(TUNEL)method was adopted to detect lacrimal gland cell apoptosis,and immunohistochemistry was used to determine the protein expression of Caspase-3,Fas,and Bcl-2 in the lacrimal gland cells.Result Compared with the normal group,the model group rabbits had decreased SⅠT,shortened TBUT,and increased CFS score(P＜0.05),with more serious pathological damage in the lacrimal gland tissues and more significant lacrimal gland cell apoptosis;the protein expression of Caspase-3 and Fas increased,and the Bcl-2 protein expression decreased in the model group(P＜0.05).Compared with the model group,the EA group rabbits showed improvements in the SⅠT,TBUT,and CFS score(P＜0.05),with reduced pathological damage in the lacrimal gland tissues and lacrimal gland cell apoptosis;the protein expression of Caspase-3 and Fas decreased,and the Bcl-2 protein expression increased in the EA group(P＜0.05).The sham EA group showed no significant differences from the model group in the above indexes(P＞0.05).Conclusion EA can improve the ocular surface function of dry eye rabbits,inhibiting lacrimal gland cell apoptosis,down-regulating the protein expression of Caspase-3 and Fas,and up-regulating the protein expression of Bcl-2 in the lacrimal gland cells.关键词
针刺疗法/电针/干眼症/细胞凋亡/兔Key words
Acupuncture therapy/Electroacupuncture/Dry eye syndromes/Cell apoptosis/Rabbits分类
医药卫生引用本文复制引用
杨光,马晓芃,王雪君,刘婕,杨延婷,张丹,刘力,卢云琼,曹姚佳妮,魏邦基..电针对干眼兔泪腺细胞凋亡及相关蛋白表达的影响[J].上海针灸杂志,2023,42(12):1314-1324,11.基金项目
上海市自然科学基金项目(22ZR1458500,21ZR1460100) （22ZR1458500,21ZR1460100）
上海市卫生健康委员会项目(20214Y0116,202040249) （20214Y0116,202040249）
