森林工程2024，Vol.40Issue(1)：1-8,8.DOI:10.3969/j.issn.1006-8023.2024.01.001
不同水曲柳褐斑病病级叶片的微生物多样性
Microbial Diversity in Leaves of Different Fraxinus mandshurica Brown Spot Disease Stages
摘要
Abstract
To investigate the microbial diversity and the main pathogenic microorganisms of Fraxinus mandshurica leaves with dif-ferent degrees of brown spot disease,and to analyze the control methods to maintain the ecological safety and enhance the economic benefits of Fraxinus mandshurica plantation forests,leaves infected with brown spot disease to varying degrees at Maoershan Experimen-tal Forest Farm were collected,and the microbial community structure and species composition of Fraxinus mandshurica leaves were de-termined and analyzed using high-throughput sequencing technology.A total of 1 026 fungal operational taxonomic units(OUT)and 322 bacterial OUTs were obtained before and after the disease in the three groups of samples.Among the bacteria,the phyla Proteobac-teria and Actinobacteriota were the dominant phyla;the genera Sphingomonas,Acidibacter and Bradyrhizobium were the main dominant genera.Among the fungi,the phyla Ascomycota and Basidiomycota were the dominant phyla;the genera Vishniacozyma,Septoria,and Hannaella were the dominant genera.There were no significant differences in bacterial diversity and community structure among the three groups of samples,and significant differences in fungal diversity and community structure.It was preliminarily speculated that the Cercospora was a pathogenic bacterium,but its pathogenicity needed further study.关键词
水曲柳/褐斑病/高通量测序/尾孢菌属/菌群结构Key words
Fraxinus mandshurica/brown spot/high-throughput sequencing/cercospora/microbial community structure分类
农业科技引用本文复制引用
刘思远,申东晨,刘峥,鲁丽颖,徐恒,董爱荣..不同水曲柳褐斑病病级叶片的微生物多样性[J].森林工程,2024,40(1):1-8,8.基金项目
国家自然科学基金项目(31670494). （31670494）
