生命科学仪器2023，Vol.21Issue(3)：19-22,4.DOI:10.11967/2023210603
单细胞转录组学测序技术的发展和挑战
Development and Challenge of Single-cell Transcriptome Sequencing Technology
摘要
Abstract
Single-cell transcriptome sequencing technology provides a powerful tool for characterizing cell hetero-geneity and analyzing complex biological systems.The further development of biomedical research continuously puts forward new challenges for this technology.After a decade of rapid development,the sensitivity of this technology is gradually improved,and the throughput has taken a huge leap.Full transcriptome and full transcriptome sequencing have also been achieved.This review introduces the development of single-cell transcriptome sequencing technolo-gy,and the technical challenges faced in improving sensitivity,increasing throughput,and achieving full-length transcript sequencing and whole transcriptome sequencing.We also proposed the need to develop advanced single-cell transcriptome sequencing technology in the future,develop automated single-cell sequencing instruments,and better integrate this technology with spatial omics and multi-omics sequencing technologies.关键词
单细胞转录组测序技术/灵敏度/通量/全转录组测序/全长转录本测序Key words
single-cell transcriptome sequencing technology/sensitivity/throughput/whole transcriptome rese-quencing/full-length transcript sequencing分类
生物科学引用本文复制引用
潘雅婷,朱强远..单细胞转录组学测序技术的发展和挑战[J].生命科学仪器,2023,21(3):19-22,4.基金项目
湖州市科学技术局科技项目(2021GZ73) （2021GZ73）
