生命科学仪器2023，Vol.21Issue(3)：54-63,10.DOI:10.11967/2023210609
基于差异蛋白质组学分析氧化应激下耐辐射奇球菌的抗氧化机制
Antioxidative mechanism of Deinococcus radiodurans under oxidative stress based on differential proteomics
摘要
Abstract
Deinococcus radiodurans has the strong vitality.It is included in the Guinness World Records and praised as"the most tenacious bacteria in the world".It is also used as a model organism for the study of resistance mecha-nism.Physical and chemical effects such as ionizing radiation and strong oxidants lead to the production of a large number of reactive oxygen free radicals with highly cytotoxic effects,which indirectly cause oxidative damage to life.It brings oxidative damage to ROS,and Deinococcus radiodurans also has a strong antioxidant mechanism,but the research on its antioxidant mechanism has not been completed.In this study,differential proteomics will be used to analyze the antioxidant mechanism of Deinococcus radiodurans under oxidative stress.Based on label-free,1853 proteins were detected in Deinococcus radiodurans after H2 O2treatment,of which 80 proteins had significant differ-ences compared with those before treatment,of which 25 were significantly up-regulated and 55 were significantly down regulated.After 4 h of culture,1853 proteins were detected in Deinococcus radiodurans group,of which 58 proteins were significantly changed,38 were significantly up-regulated and 20 were significantly down regulated.Based on the bioinformatics analysis of differential proteins and comparing the types of differential proteins,we found that the differential proteins produced by H2 O2oxidation were mainly enriched in biological processes such as resistance to oxidative stress.The differential proteins produced after 4 hours of culture recovery were significantly enriched in the process of restoring the normal physiological metabolism of Deinococcus radiodurans.The analysis shows that under the action of strong oxidants,Deinococcus radiodurans produces a protective mechanism,and the translation and ribosomal structure related proteins are up-regulated to synthesize more proteins that resist oxida-tive damage.After culture is restored,the balance is restored,and the up regulation of related proteins enables Dei-nococcus radiodurans to return to normal growth more quickly.关键词
耐辐射奇球菌/氧化应激/抗氧化机制/蛋白质组学/非标记定量分析Key words
Deinococcus radiodurans/Oxidative stress/Antioxidant mechanism/Proteomics/label-free analysis分类
医药卫生引用本文复制引用
王馥丽,魏琳洋,甘巧玉,蒋辉,宋天宇,姚戈,万秀坤..基于差异蛋白质组学分析氧化应激下耐辐射奇球菌的抗氧化机制[J].生命科学仪器,2023,21(3):54-63,10.基金项目
国民核生化灾害防护国家重点实验室科研基金项目(SKLNBC2020-13) （SKLNBC2020-13）
