水生生物学报2024，Vol.48Issue(2)：185-192,8.DOI:10.7541/2023.2023.0112
雌性斑马鱼暴露于环境剂量三唑磷对亲代生殖和氧化应激及子代胚胎发育的影响
FEMALE ZEBRAFISH EXPOSED TO ENVIRONMENTAL DOSES OF TRIAZOPHOS ON PARENTA REPRODUCTION,OXIDATIVE STRESS AND EMBRYONIC DEVELOPMENT OF OFFSPRING
摘要
Abstract
Triazophos is a widely used organophosphorus insecticide.The residual amount of triazophos detected in natural water may pose a potential threat to non-target aquatic organisms.This paper aims to investigate the toxic effect of triazophos on zebrafish.In this study,3-month-old female zebrafish(Danio rerio)were continuously exposed to en-vironmentally relevant concentrations of triazophos(10 μg/L)for 21d.Subsequently,natural spawning and fertilization were performed using healthy males,and the toxicity of triazophos on both the parental females and their offspring was assessed.The assessment included measurements of female fertility,oxidative stress in parent and offspring,as well as indicators of embryonic development in the offspring.The main results were as follows:after exposure to 10 μg/L triazophosphos,the spawning capacity of parental female fish increased significantly(P＜0.05),moreover,it caused a significantly decrease in the activity of liver acetylcholinesterase(AChE)(P＜0.05),an increase in malondialdehyde(MDA)content(P＜0.05),and a significant enhancement in the activities of superoxide dismutase(SOD),glutathione peroxidase(GPx),glutathione S-transferase(GST)and carboxylesterase(CarE)(P＜0.05).The embryo fertilization rate,fertilized egg diameter,embryo heart rate and body length of newly hatched larvae were significantly lower(P＜0.05),while the malformation rate of larval fish was significantly higher(P＜0.05).However,there was no significant diffe-rence in the hatching rate(P＞0.05).The activity of AChE and SOD in larvae decreased although not significant(P＞0.05),whereas the MDA content increased significantly(P＜0.05).Furthermore,the activities of GPx and GST were significantly decreased(P＜0.05),while CarE activity was significantly increased(P＜0.05).The above findings suggest that exposure to environmental concentrations of triazophos can increase female zebrafish's fecundity,cause oxidative stress in the parenta and their offspring,and adversely affect the developmental process of the offspring embryos.关键词
三唑磷/胚胎发育/氧化应激/解毒酶/斑马鱼Key words
Triazophos/Embryonic development/Oxidative stress/Detoxifying enzyme/Danio rerio分类
资源环境引用本文复制引用
汪和祥,李瑞娇,张建禄,张春云,王立新,陈绪,梁昊,熊冬梅..雌性斑马鱼暴露于环境剂量三唑磷对亲代生殖和氧化应激及子代胚胎发育的影响[J].水生生物学报,2024,48(2):185-192,8.基金项目
中央高校基本科研业务费(2452020154) （2452020154）
大学生创新创业训练计划(G202210712113和S202310712691)资助[Supported by the Chinese Universities Scientific Fund(2452020154) （G202210712113和S202310712691）
the College Students'Innovation and Entrepreneurship Training Program(G202210712113 and S202310712691)] （G202210712113 and S202310712691）
