水生生物学报2024，Vol.48Issue(2)：211-221,11.DOI:10.7541/2023.2023.0072
华南热带水库拟柱孢藻产毒基因型的低优势及影响因子分析——以珠海市水库为例
LOW DOMINANCE OF TOXIC GENOTYPE WITHIN THE CYLINDROSPERMOPSIS RACIBORSKII POPULATION AND ITS AFFECTING FACTORS IN TROPICAL RESERVOIRS OF SOUTHERN CHINA-A CASE STUDY OF ZHUHAI CITY
摘要
Abstract
Cylindrospermopsis raciborskii is a species of tropical cyanobacterial that can be found in natural water bo-dies.It has both toxic and non-toxic,and the coexistence of these genotypes is common.The production of cylindro-spermopsin(CYN)is a significant concern due to its high toxicity to humans,and thus investigation on the distribution its toxic strain and the potential affecting factors will help to reduce the toxic risk of C.raciborskii blooms.Based on two surveys(2018 and 2022)in 20 reservoirs in Zhuhai City in tropical southern China,we tried to understand the oc-currence of toxic C.raciborskii and assess the health risk of CYN.During the two investigated periods,C.raciborskii and its toxic genotype were detected in all 40 samples by using rpoC1-based qPCR analysis and in 33 samples with cyrJ-based qPCR.The ratios of toxic genotype accounted for 0.046％—38.66％of the total C.raciborskii abundances,indica-ting that toxic C.raciborskii was widely distributed in Zhuhai reservoirs but had low dominance in the C.raciborskii population.Compared with the year 2018,the averaged abundance of C.raciborskii in the 20 reservoirs increased nearly 10 times in 2022,however there was no significant difference in abundances between the two years.There was a significant reduction in the ratio of toxin-producing genotypes in the population.Both the mean and maximum ratios decreased in 2022 compared to 2018,with the mean ratio declining from 14.86％to 4.17％,and the maximum ratio de-creasing from 38.66％to 17.24％.The concentrations of CYN changed with a similar decreasing pattern,with the mean concentration reducing from 0.56 to 0.19 pg/L.Mixed Effect Model showed that elevated PO4-P and decreased NO3-N may favor the growth of non-toxic C.raciborskii,and the ratios of toxic C.raciborskii increased with water tempera-ture.关键词
产毒和非产毒拟柱孢藻/拟柱孢藻毒素/荧光定量PCR/热带水库/环境因子Key words
Toxic and non-toxic Cylindrospermopsis raciborskii/Cylindrospermopsin/Real-time PCR/Tropical reservoirs/Environmental factors分类
农业科技引用本文复制引用
吴孟李,范利青,吴艳龙,秦伟,商添雄,程楠,雷腊梅..华南热带水库拟柱孢藻产毒基因型的低优势及影响因子分析——以珠海市水库为例[J].水生生物学报,2024,48(2):211-221,11.基金项目
广东省自然科学基金(2023A1515012361) （2023A1515012361）
国家自然科学基金(31770507)资助[Supported by the Natural Science Foundation of Guangdong Province(2023A1515012361) （31770507）
National Natural Science Foundation of China(31770507)] （31770507）
