水生生物学报2024，Vol.48Issue(2)：242-252,11.DOI:10.7541/2023.2023.0074
接种两株有益菌的生物絮团净水性能及饲料化潜力
WATER PURIFICATION PERFORMANCE AND FEED POTENTIAL OF BIOFLOCS INOCULATED WITH TWO BENEFICIAL BACTERIA
摘要
Abstract
In order to improve the efficiency of utilizing the new flocculent forming bacterium Pseudoduganella eburnea YN12(YN)in the production of bioflocs from aquaculture wastewater,and to utilize the flocs as feed,the feasibility of feeding the Claris fuscus was explored.The study focused on bioflocs systems,and involved the inocula-tion and formulation of activated sludge(AS),Pseudomonas eburnea YN12,and Bacillus subtilis(BS)into 7 groups(AS,YN,BS,AS+YN,AS+BS,YN+BS,and AS+YN+BS).The purpose of this experiment was to analyze the diffe-rences in floc morphology and structure,as well as to evaluate the most effective combination in terms of nitrogen re-moval efficiency and microbial community structure advantages in simulated aquaculture tail water.Finally,the opti-mal combination of bioflocs was collected,dried and ground into powder and added to commercial feed granulation.They were divided into two groups:the control group and the biofloc group,to explore the effects on the growth per-formance,feed utilization rate,muscle nutrient composition,and liver digestive and immune enzyme activities of cat-fish.The results showed that the water purification effect of YN+BS group was stable,the total nitrogen removal effect was good(89.6％),and the accumulation of ammonia nitrogen,nitrite and nitrate was low.The group exhibited favo-rable floc formation(9.0 ml/L)and had dominant denitrification bacteria(47.5％)(including Hydrogenophaga,Fla-vobacterium,Pseudoxanthomonas,Burkholderiae,Comamonas,Acinetobacter,etc.).The biofloc powder was mixed with commercial feed at a 5％ratio and used for Claris fuscus breeding after grinding and granulation.It was found that the growth performance of Claris fuscus[including survival rate:(100±0.00)％＞(95±0.00)％;weight gain:(2.81±0.35)g＞(2.52±0.52)g;lenght gain:(1.68±0.36)cm＞(1.51±0.34)cm]and feed conversion rate(55±0.03＞46±0.12)were better than those of the control group of pure commercial feed.However,amylase,immune enzymes,including catalase,su-peroxide dismutase,glutathione peroxidase and other delicious amino acids in muscle nutrition,including glutamic acid,aspartic acid,alanine and glycine,were lower than those in the control group.The bioflocs feed from YN+BS group had a good advantage in growth performance of catfish,but not in digestion ability,immune activity,and muscle nutrient composition.Therefore,it is necessary to further adjust the level of biofloc addition to optimize the feeding ef-fect of Claris fuscus.关键词
有益菌/水产养殖尾水/生物絮团/反硝化菌/絮团饲料/胡子鲶Key words
Beneficial bacteria/Aquaculture tail water/Bioflocs/Denitrifying bacteria/Floc feed/Claris fuscus分类
资源环境引用本文复制引用
张波,刘泳宏,张倩倩,虞舟,王强,肖恩荣,邱东茹,吴振斌..接种两株有益菌的生物絮团净水性能及饲料化潜力[J].水生生物学报,2024,48(2):242-252,11.基金项目
国家自然科学基金(U20A2010)资助[Supported by the National Natural Science Foundation of China(U20A2010)] （U20A2010）
