水生生物学报2024，Vol.48Issue(2)：292-303,12.DOI:10.7541/2023.2023.0161
两个奥利亚罗非鱼品系对无乳链球菌的耐受性研究
THE TOLERANCE OF TWO BLUE TILAPIA(OREOCHROMIS AUREAUS)SPECIES TO STREPTOCOCCUS AGALACTIAE
摘要
Abstract
China is the leading producer of tilapia in the world,with a production volume of 1.65 million tons in 2020.However,the tilapia industry in China is facing limitations due to streptococcosis caused by Streptococcus agalactiae,which has become one of the major factors with the increased culture density and the deterioration of culture environ-ment.Breeding new varieties of tilapia resistant to streptococcosis is an important way to mitigate the disease.Diffe-rent tilapia strains exhibit varying levels of disease resistance.Therefore,this study aimed to explore the tolerance of two blue tilapia species to S.agalactiae,Oreochromis aureus"Xia'ao No.1"(AX strain)and Egypt strain(AE strain).The survival rate was recorded at different time points.Blood and spleen samples were collected at 0,7h,24h,48h,72h,120h and 168h after infection,respectively.The serum biochemical indexes and expression of pro-inflammatory cytokines in spleen of two blue tilapia species were measured.The survival rate of AE strain(30％)was significantly higher than that of the AX strain(10％)at 7d post infection.The alanine aminotransferase(ALT),aspartate aminotrans-ferase(AST),globulin(GLO),alkaline phosphatase(AKP),superoxide dismutase(SOD),lactate dehydrogenase(LDH)and lysozyme(LZM)in the serum of two blue tilapia species increased significantly after infection,whereas trigly-ceride(TG),albumin/globulin(A/G)decreased significantly.The infection resulted in a strong inhibition of the respira-tory burst.The AE strain demonstrated significantly higher serum globulin and SOD levels compared to the AX strain at later stages of infection.The mRNA levels of pro-inflammatory cytokines in the spleen of two blue tilapia species were significantly up-regulated at 7h post infection.Moreover,the gene expression of TNF-α,IL-1β and IL-6 in spleen of AE strain was significantly lower than that observed in AX strain during the later stage of observed period.The study shows that the AE strain exhibits stronger resistance to S.agalactiae compared to the AX strain.The higher antioxidant capacity and less severe inflammation observed at later stages of infection contribute to the increased tolerance of AE strain to S.agalactiae.The results provide valuable parental resistance germplasm resources for breeding streptococ-cosis resistant hybrid tilapia.关键词
无乳链球菌/血清生化指标/促炎性细胞因子/抗病力/疾病耐受性/奥利亚罗非鱼Key words
Streptococcus agalactiae/Serum biochemical indexes/Pro-inflammatory cytokine/Disease resistance/Disease tolerance/Blue tilapia(Oreochromis aureus)分类
农业科技引用本文复制引用
李可心,祝璟琳,李大宇,邹芝英,肖炜,喻杰,陈炳霖,杨弘..两个奥利亚罗非鱼品系对无乳链球菌的耐受性研究[J].水生生物学报,2024,48(2):292-303,12.基金项目
现代农业产业技术体系建设专项(CARS-46) （CARS-46）
中国水产科学研究院淡水渔业研究中心基本科研业务费(2023JBFZ01) （2023JBFZ01）
中国水产科学研究院院级基本科研业务费-罗非鱼和鲤鱼遗传育种创新团队项目(2023TD40)资助[Supported by China Agriculture Research System(CARS-46) （2023TD40）
Central Public-interest Scientific Institution Basal Research Fund,Fisheres Freshwater Research Center,CAFS(2023JBFZ01) （2023JBFZ01）
Central Public-Interest Scientific Institution Basal Research Fund,CAFS(2023TD40)] （2023TD40）
