中国生态农业学报（中英文）2023，Vol.31Issue(12)：1896-1908,13.DOI:10.12357/cjea.20230326
生态旅游型乡村的乡土景观植物遗传多样性
Plant genetic diversity of ecotourism rural landscape
摘要
Abstract
The incorporation of rural landscape plants plays a pivotal role in the development and establishment of ecotourism.By studying the genetic diversity of plants in rural landscapes,the richness of genetic variation and the stability of the genetic structure within a population can be revealed.This serves as an important theoretical foundation for the construction of rural ecological land-scapes and biodiversity maintenance and is of great significance for the construction of beautiful villages.In this study,eight native landscape species were selected from two ecotourism villages(Changkou Village,Sanming City,Fujian Province;and Paifang Com-munity,Nanjing City,Jiangsu Province).Native rural landscape plant samples were collected from the whole village area.Phenotyp-ic characteristics were measured,and ISSR-PCR experiments were performed.Through analysis of phenotypic features and detection of molecular markers,the Shannon index and Nei's genetic diversity indexes were calculated to elucidate the levels of genetic di-versity of native landscape plant species in different areas.Cluster analysis using phenotypic features identified five types of Li-quidambar formosana,six types of Cyclobalanopsis chungii,four types of Quercus glauca,sixteen types of Zelkova serrata,seven types of Toona sinensis,ten types of Aster indicus,five types of Chrysanthemum indicum,and six types of Rubus hirsutus.The phen-otypic coefficient variation and Shannon index of the eight native landscape plant species ranged from 0.23 to 0.58,and from 1.51 to 6.74,respectively.In the total area,artificial area and natural area,the Nei's genetic diversity indexes of the eight native landscape plant species ranged from 0.240 to 0.536,0.244 to 0.540,and 0.193 to 0.367,respectively.For the eight native landscape plant spe-cies,the percentage of polymorphic loci varied from 45.00％to 100.00％,the number of alleles varied from 1.45 to 2.00,and the num-ber of effective alleles varied from 1.30 to 1.64.The results revealed that the phenotypic(Shannon index)and molecular(Nei's genet-ic diversity index)genetic diversity levels of the eight native landscape plant species were higher than the average diversity level in numerous other landscape plant species.Additionally,the rural landscape plant species exhibited abundant genetic variation.The ge-netic diversity of certain rural landscape plant species exhibited a notable degree of variability;however,there were significant differ-ences in the levels of genetic diversity observed between natural and artificial areas.In the context of rural landscape construction,it is important to prioritize the assessment of genetic diversity in rural landscape plant populations.Appropriate measures should be im-plemented to enhance the even distribution of genetic polymorphisms within the population and preserve the genetic diversity of nat-ive landscape plant species.This approach is essential to ensure the long-term stability of rural ecological landscapes.关键词
生态旅游型乡村/乡土景观植物/遗传多样性/表型Shannon指数/Nei's基因多样性指数Key words
Ecotourism village/Rural landscape plants/Genetic diversity/Phenotypic Shannon index/Nei's genetic diversity index分类
生物科学引用本文复制引用
冯致,李锴,米小琴,于咏,王义强,姚志,孙全,李萌,吴秀忠,陈大华,蒋龙,范开放,唐建路..生态旅游型乡村的乡土景观植物遗传多样性[J].中国生态农业学报（中英文）,2023,31(12):1896-1908,13.基金项目
国家自然科学基金项目(32171842)、国家重点研发项目(2019YFD11004031)和中南林业科技大学创新课题(90102-63223068)资助 The study was supported by the National Natural Science Foundation of China(32171842),the National Key Research and Development Program of China(2019YFD11004031),and the Innovation Project of Central South University of Forestry and Technology(90102-63223068). （32171842）
