寒温带针叶林优势树种倒木分解真菌群落多样性及功能差异OACHSSCDCSCDCSTPCD
Diversity and functional differences of fungal communities of the decomposing fallen logs of dominant species in cold temperate coniferous forest
为了揭示寒温带针叶林不同树种倒木分解中真菌群落的分布格局,探讨影响倒木真菌群落分布的养分驱动因子.采用Illumina MiSeq高通量测序技术和R语言等分析方法对微生物多样性数据进行信息挖掘,解析白桦(Betula platyphylla Suk.)、兴安落叶松(Larix gmelinii(Rupr.)Kuzen)、樟子松(Pinus sylvestris var.mongolica Litv.)倒木分解初期真菌群落的多样性及功能差异.研究发现,寒温带针叶林中白桦、樟子松倒木优势菌群为Basidiomycota(担子菌门)、Trichaptum(附毛菌属),兴安落叶松倒木优势菌群为Ascomycota(子囊菌门)、Acidea菌属.多样性分析表明,不同树种倒木真菌群落的Alpha多样性由高到低依次为兴安落叶松、白桦、樟子松,树种的差异极显著的改变了操作分类单元(OTU)、属水平上倒木真菌群落的Beta多样性.菌群生态分析表明,腐生型是3种倒木上优势真菌的主要生态类型,木质腐生为白桦、樟子松倒木上真菌的主要生活方式,土壤腐生为兴安落叶松真菌类群的主要生活方式.养分关联分析表明,pH、全碳(TC)、全氮(TN)、碳氮比(C/N)、半纤维素、木质素等养分指标在3种倒木间存在显著性差异,其中含水率(MC)、TC、TN、纤维素、半纤维素是影响倒木优势真菌群落分布的主要养分因素.不同树种对倒木真菌群落的富集具有差异性,这种差异带来的微生物多样性及功能变化对寒温带森林生态系统的物质循环具有重要的科学意义.
This paper aims to reveal the distribution pattern of fungal communities of the decomposing fallen logs of dominant species in cold temperate coniferous forest and explore the nutrient-driving factors affecting the distribution of the fungal communities.The Illumina MiSeq high-throughput sequencing technology and R analytical method were used to mine microbial diversity data information,then analyze the diversity and functional differences of fungal communities at the initial stage of fallen logs decomposition of Betula platyphylla Suk.,Larix gmelinii(Rupr.)Kuzen,and Pinus sylvestris var.mongolica Litv..The results showed that Basidiomycota and Trichaptum were the dominant fungal communities for Betula platyphylla Suk.and Pinus sylvestris var.mongolica Litv.,and Ascomycota and Acidea were for Larix gmelinii(Rupr.)Kuzen in cold temperate coniferous forest.The diversity analysis showed that the fungal Alpha diversity values from high to low were Larix gmelinii(Rupr.)Kuzen,Betula platyphylla Suk.,and Pinus sylvestris var.mongolica Litv..The difference of tree species has extremely significant changed the Beta diversity of fungal communities on the genus and OTU levels among fallen logs intergroups.The ecological analysis of the fungi showed that saprotroph was the main ecotype of the preponderants.Wood saprotroph was the main lifestyle of the fungi of Betula platyphylla Suk..and Pinus sylvestris var.mongolica Litv..Besides,soil saprotroph was the main lifestyle of the fungi of Larix gmelinii(Rupr.)Kuzen.In addition,there were significant differences in pH value,total carbon,total nitrogen,C/N ratio,hemicellulose,and lignin among the three fallen logs.Moisture content,total carbon,total nitrogen,cellulose,and hemicellulose were the main factors affecting the fungal distribution.The enrichment of different tree species to the fungal communities of fallen logs is different,and the microbial diversity and functional changes brought by this difference have the important scientific significance for the material cycle in the cold temperate forest ecosystem.
潘虹;崔福星;杨立宾;魏丹;付晓宇;朱道光
黑龙江省科学院自然与生态研究所,哈尔滨 150040
寒温带森林倒木分解真菌群落
cold temperate forestfallen logdecompositionfungal communities
《生态学报》 2023 (023)
9856-9866 / 11
黑龙江省省属科研院所科研业务费项目(CZKYF2021-2-C011);中央引导地方科技发展专项(ZY20B15);黑龙江省科学院科学研究基金项目(KY2023ZR03)
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