实验科学与技术2023，Vol.21Issue(5)：38-43,6.DOI:10.12179/1672-4550.20220146
海藻酸钠凝胶固定化双酶快速检测葡萄糖综合实验设计
Comprehensive Experimental Design of Immobilized Dual-enzymes in Sodium Alginate Hydrogel Beads for Rapid Detection of Glucose
摘要
Abstract
To rapidly detect glucose,the immobilized dual-enzymes cascade reactor was designed by co-immobilizing glucose oxidase(GOx)and horseradish peroxidase(HRP).The micromorphology and chemical composition of sodium alginate hydrogel were analyzed by using the scanning electron microscopy(SEM)and Fourier transform infrared spectroscopy(FTIR).Meanwhile,the immobilized dual-enzymes co-catalytic system was optimized and applied for the detection of glucose in the beverage sample.Based on the enzyme-catalyzed reaction,an efficient and specific glucose detection method was established by using the cascade reaction of co-immobilized enzymes,which provids a promising strategy to design bio-detechion experiments.关键词
海藻酸钠/葡萄糖氧化酶/辣根过氧化物酶/级联反应/固定化Key words
sodium alginate/glucose oxidase/horseradish peroxidase/cascade reaction/immobilization分类
社会科学引用本文复制引用
刘艳红,王娜,李静..海藻酸钠凝胶固定化双酶快速检测葡萄糖综合实验设计[J].实验科学与技术,2023,21(5):38-43,6.基金项目
四川大学新世纪高等教育教学改革工程第十期项目资助(SCU10259) （SCU10259）
四川大学教改项目(SCU8160) （SCU8160）
四川大学研究生教育教学改革项目(GSSCU2021093) （GSSCU2021093）
国家自然科学联合基金(U21A20308). （U21A20308）
