实验科学与技术2023，Vol.21Issue(5)：83-86,117,5.DOI:10.12179/1672-4550.20230253
高效液相色谱法检测花椒中黄酮类成分综合实验设计
Design of Comprehensive Experiments for Determination of Flavonoids in Zanthoxylum Bungeanum by High Performance Liquid Chromatography
摘要
Abstract
In order to meet the teaching needs of the course of modem analytical methods for natural products of forestry chemical industry in our school,we designed a comprehensive experiment of"high performance liquid chromatography(HPLC)for the determination of flavonoids in Zanthoxylum bungeanum Maxim from different origins"in conjunction with the construction of"double first-class"majors.The content of quercetin and kaempferol in Zanthoxylum bungeanum Maxim varieties from different origins was determined by high performance liquid chromatography(HPLC)using methanol extraction and hydrochloric acid hydrolysis,and the results showed that the analytical method was accurate and reliable,and the detection limit was 0.125 μg/g with RSD less than 2％.The teaching practice showed that the experiment deepened students'understanding of theoretical knowledge,improved the fusion of students'theoretical knowledge and practical ability,promoted the scientific literacy and comprehensive thinking ability of students,and cultivated students'creative thinking and teamwork.关键词
综合实验设计/花椒/黄酮/高效液相色谱Key words
comprehensive experiments design/zanthoxylum bungeanum/flavone/HPLC分类
化学化工引用本文复制引用
唐玉莲..高效液相色谱法检测花椒中黄酮类成分综合实验设计[J].实验科学与技术,2023,21(5):83-86,117,5.基金项目
中南林业科技大学教学研究项目(2022-65). （2022-65）
