天津科技大学学报2023，Vol.38Issue(6)：12-18,80,8.DOI:10.13364/j.issn.1672-6510.20230025
山柰酚对溴氰菊酯诱导卤虫氧化应激的保护作用
Protective Effect of Kaempferol on Deltamethrin-Induced Oxidative Stress in Brine Shrimp Artemia
摘要
Abstract
In order to explore the effect of kaempferol(Ka)on the antioxidant stress of Artemia exposed to deltame-thrin(DEL),in this study we analyze the effects of DEL and Ka on the survival rate,hatching percentage,body length,antioxidant enzyme activities,lipid peroxide and Nrf2 signal pathway in Artemia parthenogenetic in Lake Ebinur,Xinjiang.The result showed that the LC50 of DEL to Artemia was 0.210µg/L,and 0.1 mg/mL Ka exerted its antioxidative effect on the hatching percentage,survival rate and body length of Artemia.Furthermore,Ka could significantly inhibit deltamethrin-induced ROS levels and malondialdehyde(MDA)content in Artemia,improve the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and catalase(CAT),increase the expression levels of key genes SOD,Nrf2,GST,NQO1,HO-1 and CAT in the Nrf2 signaling pathway,and decrease the expression levels of Keap1(P＜0.05).Therefore,the results of this study show that Ka can effectively alleviate the oxidative stress damage induced by DEL.The main mechanism is to activate the antioxidant enzymes activities of Artemia and regulate the expression of key genes in Nrf2 pathway.关键词
卤虫/山柰酚/溴氰菊酯/氧化应激Key words
Artemia/kaempferol/deltamethrin/oxidative stress分类
农业科技引用本文复制引用
马丹丹,隋丽英,周庆礼,郭庆彬,潘娜敏,李贞景..山柰酚对溴氰菊酯诱导卤虫氧化应激的保护作用[J].天津科技大学学报,2023,38(6):12-18,80,8.基金项目
天津市科技计划项目(22YDTPJC00370) （22YDTPJC00370）
天津市教委科研计划项目(2018KJ093) （2018KJ093）
