天津中医药2023，Vol.40Issue(12)：1609-1614,6.DOI:10.11656/j.issn.1672-1519.2023.12.17
基于MAPK/NF-κB通路探究藏红花素对幼年小鼠肺炎模型的治疗作用及分子机制
Exploring the therapeutic effect and molecular mechanism of crocin on a young mouse pneumonia model based on the MAPK/NF-κB pathway
摘要
Abstract
[Objective]To explore the therapeutic effect of crocin(CRO)on pneumonia in young mice and its relationship with mitogen activated protein kinase(MAPK)/nuclear factor-κB(NF-κB)pathway.[Methods]A young mouse pneumonia model was constructed by intraperitoneal injection of lipopolysaccharide(LPS).The mice were randomly grouped into control group,model group,DEX(positive control dexamethasone)group,CRO low-dose group,CRO medium-dose group,CRO high-dose group,and MAPK activator(Anisomycin group,CRO high-dose+Anisomycin).The wet to dry(W/D)ratio and myeloperoxidase(MPO)activity of mouse lungs were detected.Hematoxylin eosin(HE)staining was applied to observe pathological changes in lung tissue and pathological injury score was performed.Blood cell counters and Giemsa staining were applied to perform total cell,neutrophil,and white blood cell counts.Enzyme-linked immunosorbent assay(ELISA)was applied to detect levels of inflammatory cytokines in serum and alveolar lavage fluid(BALF).Kits were applied to detect malondialdehyde(MDA)and superoxide dismutase(SOD)in serum and BALF.Western blot was applied to detect the expression of MAPK/NF-κB pathway protein.[Results]Compared with the model group,the DEX group and CRO low,medium,and high dose groups showed reduced lung tissue injury,the W/D ratio,MPO activity,pathological injury score,total cells,neutrophils,white blood cell count,IL-1β,IL-6,TNF-α,MDA,p-p38 MAPK/p38 MAPK,p-NF-κB p65/NF-κB p65,p-IκBα/IκBα were obviously decreased,SOD was obviously increased(P<0.05).Anisomycin was able to reverse the therapeutic effect of high-dose CRO on pneumonia in mice(P<0.05).[Conclusion]CRO has therapeutic effects on young pneumonia mice,and its mechanism may be related to inhibiting the MAPK/NF-κB pathway.关键词
藏红花素/幼年小鼠/肺炎/MAPK/NF-κB通路Key words
crocin/young mice/pneumonia/MAPK/NF-κB pathway分类
医药卫生引用本文复制引用
董丽君,周波,杨翠玲,李志远,张永峰..基于MAPK/NF-κB通路探究藏红花素对幼年小鼠肺炎模型的治疗作用及分子机制[J].天津中医药,2023,40(12):1609-1614,6.基金项目
山东省中医药科技发展计划项目(2019-0043). （2019-0043）
