天津中医药大学学报2023，Vol.42Issue(6)：742-749,8.DOI:10.11656/j.issn.1673-9043.2023.06.11
解郁止痛方对偏头痛—抑郁共病模型大鼠行为学及脑组织中Iba1/RhoA蛋白表达的影响
Effects of antidepressant and analgesic formula on behavior and expression of Iba1/RhoA protein in brain tissue of rats with migraine-depression co-morbidity model
摘要
Abstract
[Objective]To observe the effects of the antidepressant formula on the behavior and expression of ionized calcium binding adaptor molecule 1(Ionized calcium binding adaptor molecule-1,Iba1)and neurodegenerative inhibitory factor Ras homolog A(Ras homolog gene family,member A,RhoA)protein in brain tissues of migraine-depressed rats,and to explore the molecular mechanism of its alleviation of migraine with depressive symptoms.[Methods]Fifty-four healthy male SD rats were randomly divided into blank group,model group,western medicine group,and low-dose,medium-dose,and high-dose groups of Chinese herbal medicine for relieving depression and pain.After 1 week of acclimatization feeding,all 5 groups except the blank group were given subcutaneous injection of nitroglycerin injection into the neck and chronic unpredictable stimulation to establish migraine-depression co-model rats.The experimental modeling was started with pharmacological gavage intervention.Mechanical pain thresholds were measured before replication of the migraine model,and behavioral assessments(including body weight,absenteeism,sugar water consumption,forced swimming,and novelty food suppression)were performed before,on day 10,and after the modeling treatment.At the end of the modelling treatment,the brain was extracted by perfusion and the expression of Iba1 and RhoA gene proteins in the amygdala of rat brain tissue was detected by protein blotting.[Results]Compared with the blank group,the rats in the model group showed a decrease in body weight,the level and vertical movement scores of the open-field experiment,the sugar-water preference rate,and the mechanical pain threshold(P＜0.01),and an increase in the novel food suppression-latency time and forced swimming immobility time(P＜0.01);compared with the model group,the rats in the western medicine and antidepressant formula dose groups showed an increase in body weight,the level and vertical movement scores of the open-field experiment,the sugar-water preference rate,and the mechanical pain threshold(P＜0.05,P＜0.01).were increased(P＜0.05,P＜0.01),and the novel food suppression-latency time and forced swimming immobility time were shortened(P＜0.05,P＜0.01).The results of protein blotting showed that Iba1 protein expression in the amygdala of rat brain tissue was elevated in the model group compared with the blank group,and decreased in each dose group of Western medicine and traditional Chinese medicine(TCM)compared with the model group,but none of them was statistically significant(P＞0.05).The expression of RhoA protein in the amygdala of rat brain tissue was significantly higher in the model group compared with the blank group(P＜0.01);compared with the model group,it was significantly lower in the Western medicine group and the high-dose group of TCM(P＜0.01).[Conclusion]The mechanism of action of the antidepressant formula,which can significantly improve the symptoms of migraine-depression co-morbidities in rats,may be related to the reduction of central sensitization,the inhibition of microglia activation and the influence of RhoA expression.关键词
偏头痛—抑郁/小胶质细胞/解郁止痛方/Iba1/RhoA/中枢敏化Key words
migraine-depression/microglia/antidepressant and analgesic formula/Iba1/RhoA/central sensitization分类
医药卫生引用本文复制引用
申莉铎,张慧,邵笑笑,蒋若琛,简芋鑫..解郁止痛方对偏头痛—抑郁共病模型大鼠行为学及脑组织中Iba1/RhoA蛋白表达的影响[J].天津中医药大学学报,2023,42(6):742-749,8.基金项目
中国中医科学院中医药循证能力建设项目(2019XZZX-NB010) （2019XZZX-NB010）
陕西省教育厅重点科研计划项目(21JS006) （21JS006）
陕西省创新能力支撑计划项目(2017KCT-26) （2017KCT-26）
陕西中医药大学附属医院科研课题项目(2020XZ002). （2020XZ002）
