塔里木大学学报2023，Vol.35Issue(4)：16-25,10.DOI:10.3969/j.issn.1009-0568.2023.04.003
基于COⅠ基因的5种新疆土著鱼类DNA条形码鉴定及聚类分析
DNA barcode identification and clustering analysis of five indigenous fishes species from Xinjiang based on COⅠ gene
摘要
Abstract
To explore the effectiveness of DNA barcode technology in the identification of indigenous fish species in Xinjiang and protect important germplasm resources in local fisheries,this study used DNA barcode technology to amplify the COⅠ gene sequence of Schizothoracinae and Triplophysa fish species,and compared it with the NCBI database.The species identification analysis was conducted by combining genetic distance and phylogenetic tree.The results showed that 33 individuals belonged to 4 genera and 5 species,including 13 haploids;The(G+C)mol% value in the COⅠ gene sequence is much lower than the(A+T)mol% value of 46.7%,which is 53.3%.It shows a significant anti G bias.The(G+C)mol% value at the third codon is 55.9%,which is much higher than the(G+C)mol% value at the first and second codons,and the R value(conversion/inversion)is 2.10;The average genetic distance between species(11.55±0.95)% is 43.8 times that of the intra species average and genetic distance(0.14±0.10)%,which is much greater than the"10 fold rule"proposed by Hebert;The phylogenetic tree shows that the subfamily Schizothorax biddulphi,Schizothorax eurystimus,Aspiorhynchus laticeps,and Diptychus maculatus of the subfamily Schizothorax biddulphi are divided into two branches,with different individuals of the same species independently aggregating into one branch,showing obvious monophyly;The long standing primitive loach(Triplophysa teunis)in the plateau loach genus also stands alone as a branch,and all species are distinguished.In summary,DNA barcode technology based on the COⅠ gene can effectively identify five indigenous fish species in Xinjiang.关键词
裂腹鱼属/DNA条形码/COⅠ基因/物种鉴定/遗传距离Key words
Schizothorax/DNA barcode/COⅠ gene/species identification/genetic distance分类
农业科技引用本文复制引用
强壮,赵年桦,张旦旦,秦凯瑞,聂竹兰,李立伟,钟馨,胡仁云..基于COⅠ基因的5种新疆土著鱼类DNA条形码鉴定及聚类分析[J].塔里木大学学报,2023,35(4):16-25,10.基金项目
塔里木大学校长基金创新研究团队项目"南疆裂腹鱼类种质资源与遗传育种创新研究团队"(TDZKCX202204) （TDZKCX202204）
科技部重点专项"新疆重点湖泊大型水生生物群落调查"(2022xjkk150403) （2022xjkk150403）
