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猪胸膜肺炎放线杆菌新疆株分离鉴定及生物学特性研究

张雪丽 张金江 王宏图 冷婧 赵颖婕 胡建军 敖维平

塔里木大学学报2023,Vol.35Issue(4):26-33,8.
塔里木大学学报2023,Vol.35Issue(4):26-33,8.DOI:10.3969/j.issn.1009-0568.2023.04.004

猪胸膜肺炎放线杆菌新疆株分离鉴定及生物学特性研究

Isolation,identification and characterization of swine Actinobacillus pleuropneumoniae of Xinjiang strain

张雪丽 1张金江 2王宏图 3冷婧 3赵颖婕 3胡建军 3敖维平3

作者信息

  • 1. 塔里木大学生命科学与技术学院,新疆 阿拉尔 843300
  • 2. 阿图什市动物卫生监督所,新疆 阿图什 845350
  • 3. 塔里木大学动物科学与技术学院,新疆 阿拉尔 843300
  • 折叠

摘要

Abstract

A suspected porcine contagious pleuropneumonia has occurred in a large-scale pig farm in Southern Xinjiang.In order to identify the pathogen,the corrent study conducted bacterial isolation,biochemical test,PCR test,drug sensitivity test and animal pathogenicity test on lung samples collected from the infected pigs.The results showed that a strain of infectious Actinobacillus pleuropneumoniae was isolated from this scale pig farm and identified as APP biotype Ⅰ and serovar 1 according to biological characteristics analysis and PCR.The pathogenicity test results showed that the strain was pathogenic to mice.The drug sensitivity test showed that the strain was sensitive to ciprofloxacin,ofloxacin,norfloxacin and other quinolones.The drug resistance gene was analyzed that the pathogen was identified as Actinobacillus pleuropneumoniae serovar 1 with floR gene.

关键词

胸膜肺炎放线杆菌/分离鉴定/apx基因/floR基因

Key words

Actinobacillus pleuropneumoniae/isolation and identification/apx gene/floR gene

分类

农业科技

引用本文复制引用

张雪丽,张金江,王宏图,冷婧,赵颖婕,胡建军,敖维平..猪胸膜肺炎放线杆菌新疆株分离鉴定及生物学特性研究[J].塔里木大学学报,2023,35(4):26-33,8.

塔里木大学学报

OACHSSCD

1009-0568

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