塔里木大学学报2023，Vol.35Issue(4)：34-40,7.DOI:10.3969/j.issn.1009-0568.2023.04.005
基于肠黏膜形态学的唾液乳杆菌拮抗大肠杆菌研究
Study on antagonistism of Lactobacillus salivarius against Escherichia coli based on intestinal mucosa morphology
摘要
Abstract
In order to investigate the antibacterial effect of Lactobacillus salivarius from chicken on Escherichia coli(ATCC25922),a strain of Lactobacillus salivarius was isolated and identified from the saliva of Xinjiang Black Chicken by selective medium combined with molecular biology.Oxford cup test was used to determine the antibacterial ability of Lactobacillus salivarius against Escherichia coli in vitro.Five week old SPF mice were randomly divided into blank control group(normal saline),inflammatory control group(1.5×108 CFU/mL),Lactobacillus salivarius group(1.5×108 CFU/mL)and antagonistic group(Lactobacillus salivarius + Escherichia coli).A mouse model of Escherichia coli infected by intragastric administration was established.The height of intestinal villus and the depth of crypt were observed and measured to evaluate the protective effect of Lactobacillus salivarius on the intestinal mucosa of mice and its ability to resist Escherichia coli.Vitro results showed that Lactobacillus salivarius from chicken had a good antibacterial effect on Escherichia coli.The results of histological sections showed that the Lactobacillus salivarius group had the longest villus height,the shallowest crypt depth,the highest villus/crypt ratio(P<0.01),and a large number of goblet cells arranged in order.The inflamma-tory control group had serious villus damage,disorderly arrangement of goblet cells,and increased crypt depth.The results showed that chicken Lactobacillus salivarius had a strong ability to antagonize Escherichia coli infection,could increase the velvet ratio of the intestinal mucosa,and had better protection and repair effects on the integrity and continuity of the intestinal mucosa in mice.The results of this study provide new ideas for the prevention and treatment of Escherichia coli infectious diseases.关键词
唾液乳杆菌/大肠杆菌/肠黏膜/抑菌/拮抗Key words
Lactobacillus salivarius/Escherichia coli/intestinal mucosa/bacteriostasis/antagonism分类
轻工纺织引用本文复制引用
谭鼎,李平,陈荣,郭雪峰,车丽妍,田冰,李涛..基于肠黏膜形态学的唾液乳杆菌拮抗大肠杆菌研究[J].塔里木大学学报,2023,35(4):34-40,7.基金项目
国家级大学生创新训练项目"益生菌制剂对大肠杆菌感染模型的拮抗作用"(202110757014) （202110757014）
塔里木大学校长基金科研条件项目"乳酸菌应用研究示范基地建设"(TDZKKY202203) （TDZKKY202203）
