陕西科技大学学报2024，Vol.42Issue(1)：28-36,9.
蔬菜膳食纤维基多菌群乳酸菌生物膜的制备、表征及其稳定性分析
Preparation,characterization and stability analysis of vegetable dietary fiber-based multi-flora lactic acid bacteria biofilm
摘要
Abstract
In this study,a self-designed biofilm-forming culture device was used,with 8 lactic acid bacteria probiotics as strains,MRS was compounded with cabbage,celery,cucumber and wax gourd vegetable juice as nutrient solution,and water-insoluble vegetable dietary fiber in nutrient solution as carrier for continuous dynamic culture.The results showed that the bac-terial concentration in the composite biofilm of lactic acid bacteria-vegetable dietary fiber could reach 2.24×1012 CFU/g after 7 days of incubation at 37℃.The results of microscopic characterization and flora structure analysis showed that both Lactobacillus and Pediococcus were enriched.The storage test and simulated digestion test of the obtained biofilms of lactic acid bacteria-vegetable dietary fiber were conducted,and the results showed that the viable count remained above 106 CFU/g after 6 months at 25℃and above 1012 CFU/g after simula-ted digestion.The test results can provide theoretical basis and technical support for the de-velopment of high bacterial concentration probiotics starter cultures,probiotics embedding materials and probiotics dietary supplements.关键词
乳酸菌/蔬菜膳食纤维/生物膜/培养装置/高通量测序/消化液耐受性Key words
lactic acid bacteria/vegetable dietary fiber/biofilm/culture device/high throughput se-quencing/digestive fluid tolerance分类
轻工纺织引用本文复制引用
吕嘉枥,杜倩茹,杨柳青,徐颖..蔬菜膳食纤维基多菌群乳酸菌生物膜的制备、表征及其稳定性分析[J].陕西科技大学学报,2024,42(1):28-36,9.基金项目
陕西省科技厅重点研发计划项目(2022NY-027) （2022NY-027）
