陕西科技大学学报2024，Vol.42Issue(1)：51-58,8.
麝香草酚破坏小肠结肠炎耶尔森氏菌细胞膜并抑制其生物被膜的形成研究
Study on the disruption of cell membrane of Yersinia enterocolitica and inhibition of its biofilm formation by thymol
摘要
Abstract
Yersinia enterocolitica is a common spoilage bacterium in the food industry,and its biofilm is a major source of contamination.In order to elucidate the mechanism of inhibition of Y.enterocolitica,the minimum inhibitory concentration(MIC),minimum inhibitory bio-film concentration(MBIC)and minimal biofilm eradicate concentration(MBEC)were first determined,followed by the cell membrane potential and the changes of intracellular ATP and p H,and finally under laser confocal microscopy(CLSM),field emission scanning elec-tron microscopy(FEGSEM)and light microscopy,Thymol-induced cell membrane damage and its inhibitory effect on biofilm were directly observed.The results illustrated that thymol had a MIC of 0.156 25 mg/mL against Y.enterocolitica and only 1/16 MIC for MBIC,which changed cell morphology and led to decreased intracellular ATP,membrane depolarization,and pH decrease.This paper aims to explore the mechanism of thymol inhibiting Y.enterocolitica and its bio-film,so as to provide more reference for whether thymol can be used as a plant-derived natural pre-servative.关键词
麝香草酚/小肠结肠炎耶尔森氏菌/生物被膜/细胞膜完整性Key words
thymol/Yersinia enterocolitica/biofilm/cell membrane integrity分类
轻工纺织引用本文复制引用
高畅,田露,卢家兴,龚国利..麝香草酚破坏小肠结肠炎耶尔森氏菌细胞膜并抑制其生物被膜的形成研究[J].陕西科技大学学报,2024,42(1):51-58,8.基金项目
陕西省教育厅专项科研计划项目(21JK0541) （21JK0541）
陕西省咸阳市秦创原科技创新专项项目(2021 ZDZX-NY-0007) （2021 ZDZX-NY-0007）
