现代妇产科进展2023，Vol.32Issue(12)：894-903,10.DOI:10.13283/j.cnki.xdfckjz.2023.12.004
基于肿瘤相关巨噬细胞的子宫内膜癌预后模型建立和验证
Integration of scRNA-Seq and TCGA RNA-Seq analyses to establish a novel risk prog-nostic model based on genes related to tumor-associated macrophages in endometrial carcinoma
摘要
Abstract
Objective:Previous studies revealed that tumor-associated macrophages(TAMs)are involved in the progression of endometrial carcinoma(EC).From this perspec-tive,we explored EC models and prognostic biomarkers.Methods:We derived single-cell se-quencing(scRNA-seq)and RNA-seq datasets of EC patients from public databases.Between tumors and healthy tissues,differentially expressed genes in tissues(DEGs)and in macropha-ges(Macrophage DEGs)were detected.To identify macrophage-associated hub genes,a Weigh-ted Gene Co-expression Network Analysis(WGCNA)was implemented.To uncover EC biomar-kers,intercrossed genes were filtered by multiple algorithms.Based on these biomarkers,risk scores were calculated,allowing EC sample division into high-and low-risk groups.Risk scores and clinical features were assessed in univariate and multivariate Cox analyses,providing inde-pendent prognostic factors for nomogram construction.Result:805 macrophage DEGs,5691 ad-ditional DEGs,733 hub genes,and 43 common genes were identified.Furthermore,six biomark-ers,including FBP1,LTB,PTGER4,PARVG,CCR7,and HLA-DMB,were filtered and risk models were constructed.Finally,to generate a EC nomogram,the risk score,age,and tumor stage were identified.Conclusions:We identified six TAM-related prognostic biomarkers and constructed a risk model,which could provide a basis for EC treatment and study.关键词
子宫内膜癌(EC)/肿瘤相关巨噬细胞(TAMs)/预后模型/单细胞测序(scRNA-seq)/加权基因共表达网络分析(WGCNA)Key words
Endometrial Carcinoma(EC)/Tumor-associated macrophages(TAMs)/Prognostic model/Single-cell sequencing(scRNA-seq)/Weighted Gene Co-expression Network Analysis(WGCNA)分类
医药卫生引用本文复制引用
何心勤,张霞,陈丽红,江洪..基于肿瘤相关巨噬细胞的子宫内膜癌预后模型建立和验证[J].现代妇产科进展,2023,32(12):894-903,10.基金项目
福建省自然科学基金(No:2023J01621) （No:2023J01621）
福建省卫生健康省级财政补助项目(No:BPB-2022HXQ) （No:BPB-2022HXQ）
