现代妇产科进展2023，Vol.32Issue(12)：924-929,6.DOI:10.13283/j.cnki.xdfckjz.2023.12.009
他克莫司调节YAP/TAZ信号通路对卵巢癌细胞增殖、凋亡和侵袭的影响
Impacts of tacrolimus on proliferation,apoptosis and invasion of ovarian cancer cells by regulating YAP/TAZ signaling pathway
摘要
Abstract
Objective:To investigate the impacts of tacrolimus(FK506)on the prolif-eration,apoptosis and invasion of ovarian cancer(OC)cells by regulating the Yes-associated protein(YAP)/transcriptional co-activator with PDZ-binding motif(TAZ)signal pathway.Methods:SKOV3 cells in logarithmic growth phase were divided into control group,negative control group(si-NC)(transfected with si-NC plasmid),silenced YAP vector group(transfect-ed with si-YAP),overexpressed negative control(pcDNA-NC)group(transfected with pcDNA-NC),and overexpressed YAP plasmid(pcDNA-YAP)group(transfected with pcDNA-YAP),FK506 group(500μg/L),FK506+si-NC group(500 μg/L FK506+transfected with si-NC plasmid),FK506+si-YAP group(500μg/L FK506+transfected with si-YAP),FK506+pcDNA-NC group(500 μg/L FK506+transfected with pcDNA-NC),and FK506+pcDNA-YAP group(500μg/L FK506+transfected with pcDNA-YAP).CCK-8 method was applied to detect cell vi-ability,flow cytometry was applied to detect cell apoptosis,Transwell experiment was applied to detect cell invasion,qRT-PCR was applied to detect YAP and TAZ mRNA expression,Western blot was applied to detect the expression levels of YAP,TAZ,matrix metalloproteinase-2(MMP-2),Bcl2 associated X protein(Bax),and CyclinD1.Results:Compared with IOSE80,the mR-NA and protein expression levels of YAP and TAZ in SKOV3,OVCAR3 and UWB1.289 cells were obviously increased(P＜0.05).Compared with si-NC group,the expression levels of YAP and TAZ mRNA and protein,cell viability and number of invasive cells in si-YAP group were significantly decreased(P＜0.05),and the apoptosis rate was significantly increased(P＜0.05).Compared with pcDNA-NC group,the expression levels of YAP and TAZ mRNA and protein,cell viability and number of invasive cells in pcDNA-YAP group were significantly in-creased(P＜0.05),and the apoptosis rate was significantly decreased(P＜0.05).Compared with the control group,the cell viability,invasion number,and the expressions of YAP,TAZ mRNA,YAP,TAZ,CyclinD1,MMP-2 in FK506 group were obviously reduced,the apoptosis rate and the expression of Bax was obviously increased(P＜0.05).Compared with FK506+si-NC group,the cell viability,invasion number,and the expressions of YAP,TAZ mRNA,the ex-pressions of YAP,TAZ,CyclinD1,MMP-2 protein in FK506+si-YAP group were obviously re-duced,the apoptosis rate and the expression of Bax were obviously increased(P＜0.05).Com-pared with FK506+pcDNA-NC group,the cell viability,invasion number,and the expressions of YAP,TAZ mRNA,the expressions of YAP,TAZ,CyclinD1,MMP-2 protein in FK506+pcDNA-YAP group were obviously increased,the apoptosis rate and the expression of Bax were obvious-ly reduced(P＜0.05).Conclusion:FK506 can inhibit the proliferation and invasion of SKOV3 cells,and induce its apoptosis by inhibiting YAP/TAZ signal pathway.关键词
他克莫司/YAP/TAZ信号通路/卵巢癌/增殖/凋亡/侵袭Key words
Tacrolimus/YAP/TAZ signal pathway/Ovarian cancer/Proliferation/Ap-optosis/Invasion分类
医药卫生引用本文复制引用
李冬梅,孟维合,康聪..他克莫司调节YAP/TAZ信号通路对卵巢癌细胞增殖、凋亡和侵袭的影响[J].现代妇产科进展,2023,32(12):924-929,6.基金项目
河北省中医药管理局科研计划项目(No:2023287) （No:2023287）
