现代科学仪器2023，Vol.40Issue(6)：195-199,5.
全内反射荧光显微镜的使用研究
Study on the use of total internal reflection fluorescence microscope
摘要
Abstract
Total internal reflection fluorescence microscope produces images by using the evanescent wave to excite the fluorescent material within 200 nm under the critical interface.It has the advantages of high signal-to-noise ratio,fast speed and little damage to biological samples.It is a commonly used single molecule fluorescence imaging technology.Based on the introduction of the principle,composition,types,advantages and disadvantages of total internal reflection fluorescence microscope,this paper fully excavates our experience in the use of total internal reflection fluorescence microscope.Taking Clathrin labeled with fluorescent protein as the research object,the application of total internal reflection fluorescence microscopy in the detection of cell membrane related molecules was described,the latest development of the instrument is discussed and the future development was prospected.We hope the paperwill provide some good suggestions for the managers and users of total internal reflection fluorescence microscope.关键词
全内反射荧光显微镜/原理/操作规程/应用Key words
Total internal reflection fluorescence microscope/Principal/Operation instruction/Application分类
医药卫生引用本文复制引用
方三华,杨丹,刘丽,肖桂凤,尹伟,刘双双..全内反射荧光显微镜的使用研究[J].现代科学仪器,2023,40(6):195-199,5.基金项目
浙江省基础公益研究计划(LGC22H310001)和浙江大学实验技术研究项目(SJS202015) （LGC22H310001）
