畜牧兽医学报2023，Vol.54Issue(12)：5046-5055,10.DOI:10.11843/j.issn.0366-6964.2023.12.015
香猪MAP3K4基因结构变异多态性和基因表达研究
Study of the Polymorphisms of Structural Variation and the Expression of MAP3K4 gene in Xiang Pig
摘要
Abstract
Based on previous data from comparative genomics,a structural variation(SV)with 189 bp in length was identified from the intron 15 of mitogen-activated protein kinase kinase ki-nase 4(MAP3K4)gene in Xiang pig,which was defined as MAP3K4-I15-sv189.To explore the SV polymorphism in pig populations and its effects on gene expression,a PCR method was estab-lished using specific primers to discriminate SV genotypes and the gene expression level was de-termined via RT-qPCR technology.There found two genotypes,DD and ID,at the MAP3K4-I15-sv189 locus in Xiang pig population,in which the gene without SV region was indicated as D,while the reference gene containing the SV region was termed to be gene I.Of those,the fre-quency was 97.35％of allele D in Xiang pig population.In Yorkshire pig population,three geno-types,DD,ⅠD and Ⅱ,were measured with frequency of 80％in allele D.And frequency of allele D in Xiang pigs was significantly higher than that in Yorkshire pigs(P＜0.05).The elements in the SV region were analyzed via Bioinformatics methods.The deletion of SV might lead to the loss of several repeat elements,which might change the expression of MAP3K4.A higher ex-pression level of MAP 3K4 mRNA was then tested from the lung of Xiang pigs compared with other tissues.And the highest level of MAP3K4 was found in the lung with genotype DD and the lowest from samples with genotype Ⅱ.It indicated that the deletion of SV MAP3K4-I15-sv189 could accelerate the expression of MAP3K4 in pig lung.The results illustrated that the SV MAP3K4-I15-sv189 in MAP3K4 gene was dominated with genotype of deletion which resulted in the increase of gene expression in the lung,and would benefit to better undestanding the mecha-nism of susceptibility to diseases in Xiang pig lung.关键词
香猪/结构变异/MAP3K4基因/多态性/肺Key words
Xiang pig/structural variation/MAP3K4 gene/polymorphism/lung分类
农业科技引用本文复制引用
徐婷婷,齐芬芳,黄世会,牛熙,李升,冉雪琴,王嘉福,谢健..香猪MAP3K4基因结构变异多态性和基因表达研究[J].畜牧兽医学报,2023,54(12):5046-5055,10.基金项目
国家自然科学基金(31960641) （31960641）
贵州省科技创新人才团队项目(黔科合平台人才[2019]5615) （黔科合平台人才[2019]5615）
贵州省"百"层次创新型人才培养项目(黔科合人才[2016]4012号) （黔科合人才[2016]4012号）
贵州省科技计划项目(黔科合基础-ZK[2021]一般103) （黔科合基础-ZK[2021]一般103）
