畜牧兽医学报2023，Vol.54Issue(12)：5077-5090,14.DOI:10.11843/j.issn.0366-6964.2023.12.018
基于转录组测序挖掘槐山羊高繁关键候选基因
Mining Key Candidate Genes for High Reproduction Performance of Huai Goats Based on Transcriptome Sequencing
摘要
Abstract
The study aimed to explore molecular regulatory mechanism and genes regulatory network of goat reproductive traits.In this study,3-year-old healthy ewes of 3rd parity with similar body size were selected.According to the reproductive performance records,3 ewes with 1 lamb on average were collected as single-lamb group,and 3 ewes with more than 2 lambs on average were collected as multi-lamb group.Transcriptome sequencing technology was used to construct mRNA library,screen differentially expressed genes(DEGs)and conduct functional enrichment analysis between single-lamb group and multi-lamb group.Real-time fluorescence quantitative PCR was used to verify gene expression.Six mRNA libraries of ovarian tissues from Huai goats were constructed,with a total number of 41 163 239 raw reads and 37 908 182 clean reads on average.A total of 121 DEGs were found between single-lamb group and multi-lamb group(P＜0.05),including 30 up-regulated genes and 91 down-regulated genes.The expressions of PTX3,MMP13,PAK1,ADAMTS1,COL1A2,CCN1,SLC4A10,FOS,NR 4A1,NR4A2 and ADCY8 genes were significantly differentially expressed between single-lamb group and multi-lamb group(P＜0.05).And these 11 DEGs were involved in the organs development and the secretion of aldosterone,cortisol,oxytocin and adrenocorticotropic hormone in the endocrine system.A total of 236 SNPs were screened in the above 11 DEGs.Seven SNPs were nonsynonymous mutations,among which,4 SNPs were detected in ADAMTS1 gene,one was in PTX3 gene,and two were in CCN1 gene.The results showed that the expression of 11 genes were significantly different between single-lamb group and multi-lamb group.These DEGs were involved tissues and organs development and hormone secretion in endocrine system.These DEGs may be related to goat follicular development,ovulation and steroid hormone secretion.The results will provide scientific basis for elucidating the genetic mechanism of high fecundity of Huai goat.关键词
槐山羊/产羔数/转录组/差异表达基因Key words
Huai goat/lambing number/transcriptome/differentially expressed genes分类
农业科技引用本文复制引用
韩浩园,李世凯,杨瑞巧,李曼曼,李君,哈斯,赵金艳,魏红芳,权凯..基于转录组测序挖掘槐山羊高繁关键候选基因[J].畜牧兽医学报,2023,54(12):5077-5090,14.基金项目
河南省重点研发与推广专项(科技攻关)(222102110024) （科技攻关）
河南省现代农业产业技术体系建设专项资金(HARS-22-15-S) （HARS-22-15-S）
