畜牧兽医学报2023，Vol.54Issue(12)：5101-5111,11.DOI:10.11843/j.issn.0366-6964.2023.12.020
运输应激不同干预方式对羔羊宰后肌肉能量代谢的影响
Effects of Different Treatments of Transportation Stress on Energy Metabolism of Lambs Muscle after Slaughter
摘要
Abstract
The purpose of this experiment was to study the effects of different treatments before and after transportation on the musle energy metabolism of lambs after slaughter,and to analyze the potential mechanism of lamb stress caused by transportation,so as to provide a reference for alleviating animal transportation stress and improving animal welfare.Sixty 4-month-old healthy Hu sheep male lambs with similar weight were randomly divided into three groups:control group,multivitamin electrolysis group and neomycin group,with 20 lambs in each group.All the experimental lambs were transported for 8 hours,and the day of transportation was recorded as the 0th day.The control group was fed with basal diet,the multivitamin electrolysis group was added with 375 mg·d-1 in the basal diet for each lamb from 2 d before transportation to 7 d after transportation,and the neomycin group was treated with neomycin 200 mg·d-1 per lamb af-ter transportation from 0 to 7 d.Five lambs in each group were slaughtered on the 0th,7th and 14th day,and the longissimus dorsi muscle was taken to detect its glycolytic potential(GP)and the expression levels of related genes and proteins.The results showed that:1)The treatment significantly affected the muscle glycogen level(P＜0.01),and the order of each group was as follows:neomycin group＞multivitamin electrolysis group＞control group.Time significantly af-fected the muscle glycogen,free glucose,hexokinase(HK)and lactate dehydrogenase(LDH)ac-tivities of the lamb(P＜0.01).The muscle glycogen and free glucose levels of the lamb at 7th and 14th day were obviously higher than that of the 0th day after transportation.The HK activity of muscle in each group increased firstly and then decreased within 0-14 d after transportation,and reached the highest on the 7th day.The muscle LDH activity increased within 14 days after transportation,reaching the highest in the control group and neomycin group on the 7th day,and the highest in the multivitamin electrolysis group on the 14th day.2)Treatment significantly af-fected the gene expression of LKB1(P＜0.05),and the order of each group was neomycin group＞control group＞multivitamin electrolysis group.Time extremely significantly affected the mRNA expression levels of AMPKα1,AMPKα2,LKB1 and CPT-1(P＜0.01),and signifi-cantly affected ACC gene(P＜0.05)as well as the gene and protein expression levels of LKB1 in lambs(P＜0.05).After transportation,the gene expression of CPT-1 and ACC in each group increased within 14 days,and the expression was the lowest on the 0th day.In summary,adding electrolytic multivitamin before and after transportation can improve muscle energy metabolism and inhibit the activation of AMPK pathway induced by transportation.关键词
羔羊/运输应激/电解多维/新霉素Key words
lambs/transportation stress/multivitamin electrolysis/neomycin分类
农业科技引用本文复制引用
陈聪慧,段春辉,杨欣雨,夏翠,郭云霞,纪守坤,严慧,刘月琴,张英杰..运输应激不同干预方式对羔羊宰后肌肉能量代谢的影响[J].畜牧兽医学报,2023,54(12):5101-5111,11.基金项目
国家肉羊产业技术体系项目(CARS-38) （CARS-38）
运输应激干预对羔羊健康及肠道菌群的影响(KY2022029) （KY2022029）
