畜牧兽医学报2023，Vol.54Issue(12)：5154-5161,8.DOI:10.11843/j.issn.0366-6964.2023.12.025
B亚型禽偏肺病毒对黄羽肉鸡致病性分析及其灭活疫苗免疫效果评价
Pathogenicity of Avian Metapneumovirus Subtype B on Yellow Feather Broilers and Evaluation on Immune Effect of Inactivated Vaccine
摘要
Abstract
To evaluate the protective efficacy of the inactivated vaccine against avian metapneumo-virus(aMPV)subtype B in yellow-feathered broilers,the inactivated vaccine LN16-I strain pre-pared in this study was immunized by intramuscular injection at a dose of 0.5 mL per chicken to yellow-feathered broilers at the age of 3 weeks.Three weeks after immunization,the same injec-tion method and dose were used once to reinforce the immunization.Serum was collected within 1-6 weeks and ELISA antibody and neutralizing antibody titers were measured.The results showed that the average ELISA antibody titer in the vaccinated group can reach 4.2 × 104 post-priming immunization,the average neutralizing antibody titer reached 7.42 log2,and the positive rate was 100％.Three weeks after the booster immunization,the chickens in each group were challenged with virulent virus LN16 at a dose of 5 000 TCID50 per chicken.The results showed that the chickens in the challenge control group had clinical symptoms such as cloudy or viscous nose,head shaking,and the incidence rate was 85％(11/13),while none of the chickens in the vaccinated group showed clinical symptoms.The number of virus copies in nasal swabs was fur-ther detected by RT-qPCR.The result showed that the control group chickens exhibited virus shedding,which reached a maximum at 3 days post-challenge,and the number of virus copies was 4.9×106 copies·mL-1,while the amount of virus shedding in the vaccinated group de-creased by 99.43％compared with that in the control group.The pathological results showed that the turbinate and trachea of the chickens in the control group showed obvious pathological damage,while no obvious pathological changes were found in the vaccinated group.The results of this study show for the first time that the infection with aMPV subtype B can cause obvious disease in yellow-feathered broilers,and the inactivated vaccine LN16-I strain has a good immune protection effect on yellow-feathered broilers.This study provides theoretical support and techni-cal guidance for the epidemiological investigation and effective prevention of aMPV subtype B in yellow-feathered broilers in China.关键词
B亚型禽偏肺病毒/灭活疫苗/黄羽肉鸡/致病性/免疫保护Key words
avian metapneumovirus subtype B/inactivated vaccine/yellow-feathered broiler/pathogenicity/protective efficacy分类
畜牧业引用本文复制引用
孟令宅,刘长军,祁小乐,吴志强,高玉龙,陈春丽,于蒙蒙,王占新,王素艳,刘鹏,何塔娜,郭茹,陈运通..B亚型禽偏肺病毒对黄羽肉鸡致病性分析及其灭活疫苗免疫效果评价[J].畜牧兽医学报,2023,54(12):5154-5161,8.基金项目
国家肉鸡产业技术体系(CARS-41-G15) （CARS-41-G15）
黑龙江省自然科学基金(TD2019C003) （TD2019C003）
