畜牧兽医学报2023，Vol.54Issue(12)：5228-5239,12.DOI:10.11843/j.issn.0366-6964.2023.12.032
沙门菌噬菌体SP3的生物学特性及基因组分析
Biological Characteristics and Genome Analysis of Salmonella Phage SP3
摘要
Abstract
This study aimed to isolate virulent Salmonella phages and provide a new biological agent for the prevention and control of bacterial diseases.The phage with Salmonella as host was isolated,and then we investigated the biological characteristics and genome features.The result showed that a virulent phage named SP3 was isolated,which charactered with a regular polyhed-ron head with approximately 70 nm in diameter and a long contractile tail with 130 nm in length.SP3 was a broad-spectrum phage that can lysate a total of 8 Salmonella strains in 3 serotypes.In addition,the optimal MOI of SP3 was 0.000 1,the incubation period was 40 min,and the out-break period was 40-160 min.We also found that SP3 is stable under the pH ranges of 4 to 11 and temperatures ≤50 ℃,respectively.The full length of phage SP3 genome was 88 039 bp,the GC content was 38.85％,and it contained 22 tRNAs and 130 ORFs,32 of which encoded proteins with known functions,but did not carry genes related to drug resistance,virulence and lysogen.SP3 was closely related to E.coli phage and Salmonella phage,both of which belonged to Ou-navirinae subfamily and Felixounavirus genus,and genomic rearrangements were also detected.The phage SP3 has strong adaptability to the environment and clear genetic background,which is expected to become an antibiotic substitute for Salmonella prevention and control.关键词
沙门菌/噬菌体/生物学特性/基因组Key words
Salmonella/phage/biological characteristics/genome分类
生物科学引用本文复制引用
韩生义,李玲霞,李淑萍,胡国元,李生庆..沙门菌噬菌体SP3的生物学特性及基因组分析[J].畜牧兽医学报,2023,54(12):5228-5239,12.基金项目
青海省基础研究计划-青年(2022-ZJ-953Q) （2022-ZJ-953Q）
