畜牧兽医学报2023，Vol.54Issue(12)：5252-5263,12.DOI:10.11843/j.issn.0366-6964.2023.12.034
基于小鼠骨骼肌损伤模型分析丹参素对骨骼肌损伤后修复再生的促进作用
The Promoting Effect Analysis of Danshensu on Skeletal Muscle Repair and Regeneration after Skeletal Muscle Injury in Mice based on a Mouse Skeletal Muscle Injury Model
摘要
Abstract
The purpose of this study was to investigate the effect of Danshensu,the monomer component of Salvia miltiorrhiza,on the repair of skeletal muscle injury,in order to provide the theoretical basis for clinical treatment.The skeletal muscle injury models were established and treated with Danshensu.The process of skeletal muscle repair was observed via H.E.and Mas-son staining.Western blot and Q-PCR were used to detect the expression of muscle regeneration related factors(α-Actinin,eMyHC,MyoD,MyoG,Pax7 and MSTN),inflammation related fac-tors(IL-6 and IL-10),macrophage type related factors(F4/80),scar tissue formation related factors(Collagen Ⅰ and α-SMA)and intermuscular adipose-related factors(PPAR-γ).The re-sults showed that Danshensu reduced Evans Blue uptake of injure gastrocnemius muscle and sig-nificantly increased the expression levels of α-Actinin and eMyHC.Meanwhile,the expression levels of muscle regeneration related factors,MyoD,MyoG and Pax7,were also significantly in-creased and MSTN was obviously decreased after adding Danshensu.Danshansu significantly re-duced the expression of F4/80 and accelerated the transformation of M1 macrophages into M2 macrophages,reducing the IL-6 mRNA level and increasing the IL-10 mRNA level.Masson staining showed that Danshensu reduced the distribution of collagen fibers.In addition,it also re-duced the expression of Collagen Ⅰ and α-SMA.Meanwhile,Danshensu significantly increased the expression of PPAR-y in muscle.Therefore,Danshensu can promote muscle fiber regeneration and the formation of intermuscular adipose,reduce the formation of scar tissue,thereby accelera-ting the process of skeletal muscle repair and improving the quality of skeletal muscle repair.关键词
骨骼肌损伤与再生/丹参素/肌间脂肪/炎症反应/纤维化Key words
skeletal muscle injury and regeneration/Danshensu/intermuscular adipose/inflam-matory response/fibrosis分类
农业科技引用本文复制引用
薛霖莉,孙睿,郝晓静,曹校瑞,王海东,卢嘉茵..基于小鼠骨骼肌损伤模型分析丹参素对骨骼肌损伤后修复再生的促进作用[J].畜牧兽医学报,2023,54(12):5252-5263,12.基金项目
山西省博士毕业生来晋工作奖励经费科研项目(SXBYKY2022013 （）
SXBYKY2022039) （）
山西省基础研究计划青年项目(202103021223166) （202103021223166）
