畜牧兽医学报2023，Vol.54Issue(12)：5275-5292,18.DOI:10.11843/j.issn.0366-6964.2023.12.036
基于UHPLC-QE-Orbitrap-MS技术的深加工槟榔籽的成分分析
Composition Analysis of Seed of Areca catechu L.in Deep Processing based on UHPLC-QE-Orbitrap-MS Technology
摘要
Abstract
To study the chemical composition of Areca catechu L.seed in deep processing,UH-PLC-QE-Orbitrap-MS technology was used to analyze and identify the composition of Areca cate-chu L.seed.Gradient elution was performed on ACQUITY UPLC HSS T3 column(2.1 mm× 100 mm,1.8 µm)with 0.1％formic acid aqueous solution(A)to 0.1％formic acid acetonitrile solution(B)as mobile phases.The flow rate was 0.3 mL·min-1,the column temperature was 35 ℃,and the sample volume was 10 μL.An electric spray ion source(ESI)was used with a scanning range of 100-1 200 m/z and positive and negative ions were collected simultaneously,and the detection mode was Full MS-ddMS2.The original data were analyzed using Compound Discoverer 3.3 software,and the structures of the compounds were identified using the local da-tabase and mzCloud online database.Eighty-five compounds of Areca catechu L.seed in deep processing were detected,including 3 alkaloids,24 flavonoids,15 organic acids,7 phenols,7 ter-penoids,6 amino acids,4 carbohydrates,4 esters,2 amides,2 sphingolipids,1 steroid,1 phe-nylpropanoid,1 coumarin and 8 other species.The UHPLC-QE-Orbitrap-MS detection technolo-gy quickly identified the compound of Areca catechu L.seed in deep processing,and the research results provided scientific basis for exploring the application prospects and developing high value-added products of Areca catechu L.关键词
槟榔籽/副产物/超高效液相色谱-四极杆-静电场轨道肼高分辨质谱法/成分Key words
the seed of Areca catechu L./by-product/UHPLC-QE-Orbitrap-MS/composition分类
农业科技引用本文复制引用
马丹阳,高雪嫣,彭凌峰,伍少峰,王庆涛,郝智慧..基于UHPLC-QE-Orbitrap-MS技术的深加工槟榔籽的成分分析[J].畜牧兽医学报,2023,54(12):5275-5292,18.基金项目
三亚崖州湾科技城管理局资助(SYND-2022-01) （SYND-2022-01）
