畜牧兽医学报2023，Vol.54Issue(12)：5312-5317,6.DOI:10.11843/j.issn.0366-6964.2023.12.039
1-甲基海因对慢性疼痛大鼠钠离子通道蛋白Nav1.8的干预作用
Effect of 1-methylhydantoin on Sodium Channel Nav1.8 in Rats with Chronic Pain
摘要
Abstract
The purpose of this study is to investigate the effect of 1-methylhydantoin on Nav Channels 1.8,which can provide a theoretical basis for pain intervened and different ways to a-chieve the ultimate goal of"pain minimization".Sixty SPF SD rats were randomly divided into six groups.The group was treated with 1-methylhyne(group MH),lidocaine(group L)and am-broxol(group A),respectively.On the 21st and 28th day after modeling,Western blot was used to detect the protein expressions of Nav1.8 in the L4-L6 dorsal root ganglia of rats,and patch-clamp technique was used to detect the peak currents of Nav1.8 channels under the effect of MH.Comparing with the group of model,the results showed that MH could decrease the expression of Nav1.8 significantly(P＜0.05).MH inhibited the channel peak currents of Nav1.8 to a certain extent.The current inhibition rates of 10 µmol·L-1 and 100 μmol·L-1 MH to Nav1.8 were 6.34％+3.13％and 14.6％±1.52％respectively,which is showing a dose-response relation-ship.It is suggested that MH can decrease the expression of Nav1.8 in DRG of rats with chronic pain,and inhibit Nav1.8 current at the same time,thus producing analgesic.关键词
1-甲基海因/大鼠/慢性疼痛/Nav1.8/膜片钳技术Key words
1-methylhydantoin/rat/chronic pain/Nav1.8/patch-clamp technique分类
农业科技引用本文复制引用
梁雾滢,刘镇,曾玉淇,吕俊瑾,莫睿文,远立国..1-甲基海因对慢性疼痛大鼠钠离子通道蛋白Nav1.8的干预作用[J].畜牧兽医学报,2023,54(12):5312-5317,6.基金项目
广东省自然科学基金(2013B040200032) （2013B040200032）
