心脑血管病防治2023，Vol.23Issue(11)：27-31,5.DOI:10.3969/j.issn.1009-816x.2023.11.006
人巨细胞病毒感染与冠心病患者Treg/Th17平衡及miR-155表达的关系
Relationship between human cytomegalovirus infection and regulatory T cells/helper T cells 17 balance and microRNA-155 expression in patients with coronary heart disease
摘要
Abstract
Objective To analyze the correlation between human cytomegalovirus(HCMV)infection and the balance of regulatory T cell(Treg)/helper T cell 17(Th17),microRNA-155(miR-155)expression in peripheral blood of patients with coronary heart disease(CHD),and to explore the role of HCMV infection in the occurrence and development of CHD.Methods A total of 160 inpatients with CHD admitted to the cardiology department of the Fourth Central Hospital of Baoding City from January 2018 to January 2019 were selected,including 84 cases of acute coronary syndrome(ACS)as the ACS group and 76 cases of stable angina pectoris(SAP)as the SAP group.In addition,80 non-CHD patients underwent physical examination during the same period in the hospital were selected as the control group.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum levels of HCMV-IgG,HCMV-IgM,interleukin-10(IL-10),interleukin-17(IL-17)and transforming growth factor-β(TGF-β).Real-time fluorescent quantitative PCR(RT-qPCR)was used to detect serum miR-155 expression.Flow cytometry was used to detect the proportions of Th17 cells and Treg cell subsets in peripheral blood,and calculate the ratio of Treg/Th17.Pearson analysis was used to evaluate the correlation between serum HCMV-IgM and miR-155,Treg/Th17 in ACS patients.Results Compared with the control group,the positive rate and level of HCMV-IgM,the positive rate and level of HCMV-IgG,Th17 proportion,and IL-17 level in the SAP group and the ACS group increased,the levels of serum miR-155,IL-10,TGF-β,Treg proportion,and Treg/Th17 ratio reduced,the differences were statistically significant among the three groups(χ2/F=35.744,38.400,36.526,151.238,114.878,439.705,185.429,384.825,127.762,53.129,533.803;all P<0.05).Compared with the SAP group,the positive rate and level of HCMV-IgM,Th17 proportion,and IL-17 level in the ACS group increased,the levels of serum miR-155,IL-10,TGF-β,Treg proportion and Treg/Th17 ratio reduced,the differences were statistically significant(all P<0.05).In the ACS group,the serum miR-155 level,Treg proportion,and Treg/Th17 ratio of HCMV-IgM-positive patients were lower than those of HCMV-IgM-negative patients,and the Th17 proportion was higher than that of HCMV-IgM-negative patients,the differences were statistically significant(t=-9.430,-3.127,-5.005,2.366;all P<0.05).Correlation analysis showed that serum HCMV-IgM in ACS patients was negatively correlated with miR-155 and Treg/Th17 ratio(r=-0.647,-0.569;P<0.05).Conclusion HCMV infection may be involved in the occurrence and development of CHD,which is related to the serum miR-155 and Treg/Th17 ratio in patients with CHD.关键词
人巨细胞病毒/调节性T细胞/辅助性T细胞17平衡/冠心病/微小RNA-155Key words
Human cytomegalovirus/Regulatory T cell/helper T cell 17 balance/Coronary heart disease/MicroRNA-155引用本文复制引用
郑晓拓,田红彪,王玉肖..人巨细胞病毒感染与冠心病患者Treg/Th17平衡及miR-155表达的关系[J].心脑血管病防治,2023,23(11):27-31,5.基金项目
河北省保定市科学技术局基金项目(2041ZF041) （2041ZF041）
