新医学2023，Vol.54Issue(12)：864-871,8.DOI:10.3969/j.issn.0253-9802.2023.12.005
不同无血清培养体系对人脐带间充质干细胞生物学功能的影响
Effects of different serum-free culture systems on the biological function of hUC-MSCs
摘要
Abstract
Objective To evaluate the effects of three different serum-free culture systems on the biological function of human umbilical cord mesenchymal stem cells(hUC-MSC)cultured in vitro.Methods Three strains of umbilical cord tissues were obtained,divided into 3 experimental groups and cultured to the 3rd generation(P3)in three different serum-free culture systems(Group A:serum-free culture system synthesized by pure chemical components,group B:serum-free culture system consisting of platelet lysate+basic culture medium,group C:serum-free culture system consisting of fibrinogen coating+recombinant human protein).The cell proliferation,cell phenotype,three-line differentiation and immunomodulatory function were compared.Results All the cells in three groups were long and flat fusiform,adherent to the wall and uniform in size.The proliferation curve trend was similar,and the proliferation ability of P2 and P3 generations in groups A and B was better than that in group C(both P<0.05).The cells in three experimental groups all met the requirements of the International Stem Cell Society for identification of hUC-MSC cell surface markers.The expression levels of CD29 and CD90 in P3 generations in groups A and B were higher than those in group C(all P<0.05),and all three experimental groups had the ability of three-line differentiation.Compared with the control group,the proliferation rate of T lymphocytes in P3 generation and the proportion of cytokines secreted by Th1 and Th17 cell subsets were significantly decreased in the three experimental groups,and the proportion of regulatory T cells(CD4+ CD25+ FOXP3+)was significantly increased in the three experimental groups,but there was no statistical significance among three experimental groups(all P>0.05).Conclusions The hUC-MSC in three groups can maintain basic biological characteristics and immunomodulatory activity.The proliferation ability and expression levels of cell surface markers in groups A and B are superior to those in group C.关键词
人脐带间充质干细胞/无血清培养体系/生物学功能Key words
Human umbilical cord mesenchymal stem cell/Serum-free culture system/Biological function引用本文复制引用
林惠珠,李翠平,陈晓燕,阳莉..不同无血清培养体系对人脐带间充质干细胞生物学功能的影响[J].新医学,2023,54(12):864-871,8.基金项目
广东省药品监督管理局科技创新项目(2021TDB22) （2021TDB22）
