新医学2023，Vol.54Issue(12)：872-878,7.DOI:10.3969/j.issn.0253-9802.2023.12.006
壳聚糖/BMP-2质粒温敏水凝胶复合体促犬牙槽骨再生的研究
The promoting effect of chitosan thermosensitive hydrogel complex with chitosan nanoparticles carrying BMP-2 plasmid DNA on alveolar bone regeneration in beagle dogs
摘要
Abstract
Objective To construct the chitosan/BMP-2 plasmid thermosensitive hydrogel complex CS/CSn(pDNA-BMP2)-GP,and evaluate the effect of this composite system on alveolar bone regeneration in beagle dogs.Methods Periodontitis models were established in the second and third premolars from 4 beagle dogs.All teeth were randomly divided into three groups:CS/CSn-GP group,CS/CSn(pDNA-BMP2)-GP group and blank control group.In the CS/CSn-GP group,CS/CSn-GP was injected.In the CS/CSn(pDNA-BMP2)-GP group,CS/CSn(pDNA-BMP2)-GP was injected.In the blank control group,no material was injected.All animals were sacrificed 8 weeks later.The alveolar bone regeneration in periodontitis was observed by Masson staining.The expression of alkaline phosphatase(ALP)in bone defects was observed by calcium and cobalt staining.Results Masson staining showed that different degrees of alveolar bone regeneration was noted in the CS/CSN-GP and CS/CSn(pDNA-BMP2)-GP groups,and significant regeneration was noted in the CS/CSn(pDNA-BMP2)-GP group.Calcium and cobalt staining revealed strongly-positive ALP in the CS/CSn(pDNA-BMP2)-GP group,weakly-positive ALP in the CS/CSN-GP group,and negative result in the blank control group.The average optical density value in the ALP-positive staining area in the CS/CSn(pDNA-BMP2)-GP group was higher than that in the CS/CSn-GP group,followed by that in the blank control group(all P<0.05).Conclusions CS/CSn-GP composite repair system exerts positive effect on alveolar bone regeneration.Inclusion of pDNA-BMP2 can significantly promote the effect on alveolar bone regeneration.关键词
牙周炎/壳聚糖纳米粒/组织工程支架/骨形态蛋白2/温敏水凝胶Key words
Periodontitis/Chitosan nanoparticle/Tissue engineering scaffold/BMP-2/Thermosensitive hydrogel引用本文复制引用
李慧,吉秋霞..壳聚糖/BMP-2质粒温敏水凝胶复合体促犬牙槽骨再生的研究[J].新医学,2023,54(12):872-878,7.基金项目
国家自然科学基金青年科学基金(81401526) （81401526）
