云南化工2023，Vol.50Issue(12)：55-59,5.DOI:10.3969/j.issn.1004-275X.2023.12.14
基于衍生化UPLC法测定不同产地天麻中19种游离氨基酸含量
Determination of 19 Free Amino Acids in Gastrodia Elata from Different Origins by UPLC after Derivatization
摘要
Abstract
6-Aminoquinoline-N-hydroxysuccinimide carbamate was used as the derivative reagent to establish a derivative UPLC method for the determination of 19 kinds of free amino acids in G.elata.The quality differences of G.elata from different origins were analyzed.The linear relationships of the 19 free amino acids were good in a certain concentration range,the correlation coefficients were all greater than 0.996,the reproducibility RSDs were all less than 3.0%,and the recoveries were 91.99%-114.59%,which indicated the method was accurate and reli-able.There are obvious differences in the content of free amino acids in G.elata from different origins.The total content of 19 free amino acids in G.elata from different origins is in the range of 362.12-644.95 mg/100 g.The average value of the total free amino acids in G.elata from Zha-otong Yunnan Province(517.41 mg/100 g)>that out-of-Yunnan Province(505.68 mg/100 g)>those of other origins in Yunnan Province(444.56 mg/100mg).The experimental method wasaccurate,rapid and efficient,which can provide scientific basisfor decision analysisof G.elata.The study can provide reference for the further exploration and evaluation of the intrinsic quality of G.elata from different producing areas and rational development and utilization of resources.关键词
天麻/游离氨基酸/含量测定Key words
Gastrodia elata/free amino acid/content determination分类
医药卫生引用本文复制引用
严春荣,魏建强,张海瑞,黄子玉,孙梦凡,刘祥义,侯英..基于衍生化UPLC法测定不同产地天麻中19种游离氨基酸含量[J].云南化工,2023,50(12):55-59,5.基金项目
云南省科技厅农业基础研究联合专项项目(202301BD070001-236). （202301BD070001-236）
