医学信息2023，Vol.36Issue(24)：37-41,5.DOI:10.3969/j.issn.1006-1959.2023.24.006
补骨益髓饮治疗骨质疏松大鼠的作用机理研究
Study on the Mechanism of Bugu Yisui Decoction in the Treatment of Osteoporosis Rats
摘要
Abstract
Objective To study the mechanism of Bugu Yisui decoction in the treatment of osteoporosis rats.Methods A total of 60 rats were used to establish a rat model of osteoporotic fracture.They were divided into test group,positive control group and model group by random number table method,with 30 rats in each group.The test group was given Bugu Yisui decoction by gavage,the positive control group was given testosterone by gavage,and the model group was given equal volume of distilled water.Another 20 rats were selected as the blank group.The bone mineral density of the left tibia fracture site of each group was compared,and the morphological changes of bone tissue were observed.The changes of prostaglandin E2 and prostacyclin in serum,gene and protein expression in tibia tissue were detec ted.Results The tibial tissue status of the model group was worse than that of the blank group,and the histopathological changes were serious.Compared with the blank group,PGE2 and PGI2 in the serum of the model group were increased(P＜0.05).Compared with the model group,the contents of serum PGE2 and PGI2 of rats in the test group were decreased(P＜0.05).There was no significant difference in serum PGE2 and PGI2 levels between the positive control group and the test group(P＞0.05).Compared with the blank group,the expression of BMP2 and TIMP1 protein in the tibial tissue of the model group decreased,and the expression of TIMP1 protein in the tibial tissue of the test group increased compared with the model group(P＜0.05).Compared with the positive control group,the protein expression was increased(P＜0.05).Compared with the model group,the expression of BMP2 protein in the tibia tissue of the rats in the test group was increased(P＜0.05),and there was no significant difference compared with the positive control group(P＞0.05).Compared with the blank group,the expression of BMP2 and TIMP1 genes in the tibia tissue of the model group decreased,and the difference was statistically significant(P＜0.05).The gene expression of BMP2 and TIMP1 in tibia tissue of rats in the test drug group was higher than that in the model group and the positive control group(P＜0.05),and the gene expression of BMP2 and TIMP1 in tibia tissue of rats in the positive control group was higher than that in the model group(P＜0.05).Conclusion Bugu Yisui decoction has a certain therapeutic effect on osteoporosis rat model,and the related mechanism may be related to its inhibition of serum PGE2/PGI2 level,promotion of BMP2/TIMP1 gene and protein expression,and improvement of bone quality.关键词
补骨益髓饮/骨质疏松大鼠/骨折Key words
Bugu Yisui decoction/Osteoporosis rats/Fracture分类
医药卫生引用本文复制引用
王文建,陈志勇,王博韬,李昊辰..补骨益髓饮治疗骨质疏松大鼠的作用机理研究[J].医学信息,2023,36(24):37-41,5.基金项目
天津市北辰区科学技术局·天津市北辰区卫生健康委科研项目(编号:SHGY-2020047) （编号:SHGY-2020047）
