空军军医大学学报2023，Vol.44Issue(12)：1148-1154,7.DOI:10.13276/j.issn.2097-1656.2023.12.003
机体保护多肽157对皮肤烫伤和切割伤的保护作用及安全性评价研究
Protective effect and safety evaluation of body protection compound 157 on skin scald and incision injury
摘要
Abstract
Objective To study the therapeutic effect of body protection compound 157(BPC157)on skin scald in mice and incision injury in rats.Methods The mice and rats were divided into 5 groups,including model group(injection of normal saline:0.1 mL/20 g body mass),positive control group(recombinant bovine basic fibroblast growth factor gel 300 IU/cm2),BPC157 dose groups(10,20,and 40 μg/kg).Mice were exposed to a desktop super constant temperature scald apparatus at the hair removal site for 8 s,resulting in a Ⅲ degree scald model with an area of 2 cm2.After depilation on the back of rats,a skin incision of 4 cm in length was made with a sterile scalpel parallel to the spine to construct a skin incision model.After the establishment of the model,BPC157 was continuously given for 7 d to observe the wound healing.Meanwhile,the expressions of collagen and hydroxyproline on the wound were detected by ELISA,HE staining and Masson staining were performed to explore the pathological changes,and the drug safety was evaluated in mice.Results Compared with the model group,BPC157 dose groups promoted the healing of skin scald and incision injury,and increased the expression levels of collagen and hydroxyproline,as well as subcutaneous collagen fibers.In terms of safety evaluation,there was no significant change in body mass and mass of major organs in mice after continuous treatment for 1 week,and no obvious toxic reaction.Conclusion Studies have shown that BPC157 can significantly promote the repair of skin scald and the healing of skin wound with good safety,which provides experimental basis for its wide application in the clinical treatment of skin trauma.关键词
机体保护多肽157/皮肤烫伤/切口伤/伤口愈合/安全性评价Key words
body protection compound 157/skin scald/incision injury/wound healing/safety evaluation分类
药学引用本文复制引用
李招招,张英起,何磊,杜海琛,谢飞,史佳欣,孟赛男,石格格,邱玥媛,王昭维,李萌..机体保护多肽157对皮肤烫伤和切割伤的保护作用及安全性评价研究[J].空军军医大学学报,2023,44(12):1148-1154,7.基金项目
国家自然科学基金(81603009) （81603009）
陕西省自然科学基金(2020JQ-445) （2020JQ-445）
