空军军医大学学报2023，Vol.44Issue(12)：1177-1184,8.DOI:10.13276/j.issn.2097-1656.2023.12.007
新型二氢氧杂蒽结构近红外荧光探针的合成及其在过氧化氢检测中的应用
Synthesis of a novel dihydroxanthene structure near-infrared fluorescence probe and its application in the detection of hydrogen peroxide
摘要
Abstract
Objective To design and synthesize a novel dihydroxanthene(DHX)structure near-infrared fluorescence probe for in situ,trace,visualization,and specific detection of hydrogen peroxide(H2 O2).Methods A novel mitochondrial targeted fluorescence probe molecule DHX-H2O2 was constructed with DHX structure as fluorophore and pyridine salt as acceptor.The fluorescence properties,selectivity,relationship with H2O2 concentration and recognition mechanism of the probe were studied.In addition,the application of the probe in the detection of H2O2 in cells and zebrafish were also studied.Results The probe DHX-H2 O2 could achieve near-infrared(λem=675 nm)fluorescence response to H2O2 with good selectivity and excellent sensitivity(4.21 μmol/L).In addition,the probe had the ability to locate the mitochondria of cells,with a Pearson correlation coefficient of 0.97.Conclusion The designed and synthesized probe molecule has the advantages of good selectivity,high sensitivity and low cytotoxicity,and has successfully realized the imaging detection of endogenous and exogenous H2O2 in cells and zebrafish,which has broad biological application prospects.关键词
过氧化氢/荧光探针/线粒体靶向/近红外/成像检测Key words
hydrogen peroxide/fluorescence probe/mitochondrial targeting/near-infrared/imaging detection分类
医药卫生引用本文复制引用
田秦秦,卢先林,陈佳鑫,王海波,何炜..新型二氢氧杂蒽结构近红外荧光探针的合成及其在过氧化氢检测中的应用[J].空军军医大学学报,2023,44(12):1177-1184,8.基金项目
国家自然科学基金(21272272) （21272272）
军事医学提升计划项目(2020SWAQ05) （2020SWAQ05）
陕西省自然科学基础研究计划项目(2023-JC-QN-0868) （2023-JC-QN-0868）
陕西省重点研发计划一般项目(2022SF-048) （2022SF-048）
