医药导报2023，Vol.42Issue(12)：1758-1764,7.DOI:10.3870/j.issn.1004-0781.2023.12.003
梅州客家41种常用中草药对α-葡萄糖苷酶和α-淀粉酶的抑制作用
Inhibitory Effects of 41 Common Chinese Herbal Medicines on α-glucosidase and α-amylase in Meizhou Hakka
摘要
Abstract
Objective Taking 41 kinds of Chinese herbal medicines commonly used in Meizhou Hakka as the research object,their inhibitory activities against α-glucosidase and α-amylase were screened and the enzyme inhibition types of the species with the strongest activities were explored.Methods The inhibitory activities of 41 commonly used Hakka herbs in Meizhou against α-glucosidase and α-amylase were evaluated by the p-Nitrophenyl a-D-mannopyranoside(pNPG)method and the 3,5-Dinitrosalicylic acid(DNS)method,using the inhibitory rate of half(IC50)as an index.The inhibitory activity of 95%ethanol extracts of 41 Chinese herbal medicines commonly used in Meizhou Hakka on α-glucosidase and α-amylase were analysed.The enzymatic kinetics method and Lineweaver-Burk curve were used to analyze the inhibitory type of the most active species.Results The results showed that 40 Chinese herbal medicines commonly used in Meizhou Hakka had α-glucosidase inhibitory activity,and 23 medicines had α-amylase inhibitory activity,among which Psychotria asiatica Wall.showed the strongest inhibitory activity with the IC50 values aganist α-glucosidase and α-amylase of 0.17±0.001 mg·mL-1 and 0.09±0.001 mg·mL-1,respectively.The inhibition types were reversible competitive inhibition and reversible non-competitive inhibition,respectively.Conclusion The Psychotria asiatica Wall.Chinese herbal medicines commonly used in Meizhou Hakka has significant inhibitory effect on the activity of glucose metabolism enzymes,which has potential value for further research and development on the prevention and treatment of diabetes mellitus.关键词
中草药/梅州客家/α-葡萄糖苷酶/α-淀粉酶/抑制作用Key words
Chinese Herbal Medicines/Meizhou Hakka/α-glucosidase/α-amylase/Inhibitroy effects分类
医药卫生引用本文复制引用
丘建媚,刘付薇,陈星梅,庄远杯,张声源,吴俊标..梅州客家41种常用中草药对α-葡萄糖苷酶和α-淀粉酶的抑制作用[J].医药导报,2023,42(12):1758-1764,7.基金项目
国家自然科学基金资助项目(8197140105) （8197140105）
广东省中医院拔尖人才项目(BJ2022KY15) （BJ2022KY15）
2022年广东省基地嘉应学院客家研究院大客家平台研究团队项目(22KYKT03). （22KYKT03）
