医药导报2023，Vol.42Issue(12)：1765-1771,7.DOI:10.3870/j.issn.1004-0781.2023.12.004
基于斑马鱼模型的鹿胶益气补血相关功效研究
Study on the Efficacy of Cervi Colla in Yiqi Buxue Based on the Zebrafish Model
摘要
Abstract
Objective To quickly observe the tonic effects of Cervi Colla on enriching blood,strengthening bones and anti-aging in zebrafish model.Methods Glyphosate(Gly)was used to construct the erythrocyte injury model in adult zebrafish,and methotrexate(MTX)was used to construct the hematopoietic function injury model of juvenile zebrafish.Prednisolone(Pred)was used to establish the inhibition model of bone formation in zebrafish larvae.The ocular cell apoptosis model of zebrafish larvae was established by dibutyl phthalate(DBP).Results Cervi Colla could improve the Gly-induced abnormal erythrocyte nucleus in adult zebrafish and promote the expression of hematopoietic factors SCL and GATA1.Cervi Colla improved Pred-induced inhibition of bone formation in juvenile zebrafish,and promoted the expression of osteoblast-related gene ALP and Runx2a.The number of ocular cell apoptosis induced by DBP was decreased,and the expression of anti-apoptotic factor Bcl-2 was promoted.Conclusion Cervi Colla has significant effects on protecting erythrocyte,protecting hematopoiesis,protecting bone formation and anti-apoptosis.These effects may be related to replenish blood,anti-osteoporosis,and anti-aging.This study provides a scientific basis for the clinical application of Cervi Colla,and lays a foundation for further development and application.关键词
鹿胶/益气补血/红细胞损伤/骨形成抑制/细胞凋亡Key words
Cervi Colla/Tonify energy and enrich the blood/Erythrocyte injury/Inhibition of bone formation/Cell apoptosis分类
医药卫生引用本文复制引用
刘雨彤,黄勇,毛洪运,张童,崔杰,李梦雨,候蓉,郑云枫,华永庆..基于斑马鱼模型的鹿胶益气补血相关功效研究[J].医药导报,2023,42(12):1765-1771,7.基金项目
贵州省科技计划项目([2020]4Y090号) （[2020]4Y090号）
贵阳市科技计划项目([2020]-18-4号) （[2020]-18-4号）
江苏省高等学校自然科学研究重大项目(20KJA360001). （20KJA360001）
