医药导报2023，Vol.42Issue(12)：1779-1784,6.DOI:10.3870/j.issn.1004-0781.2023.12.006
LC-MS/MS法同时测定血浆中氟马替尼及其代谢产物浓度
Determination of Flumatinib and Its Two Major Metabolites in Plasma by LC-MS/MS
徐月华 1钱洲逸 2赵杨 2黄琼叶 2孙鲁宁 2王永庆 2孙志明 3唐雯雯2
作者信息
- 1. 海安市人民医院药剂科,海安 226600
- 2. 南京医科大学第一附属医院药学部,南京 210029
- 3. 国家卫生健康委计划生育药品不良反应监测中心/江苏省卫生健康发展研究中心,南京 210029
- 折叠
摘要
Abstract
Objective A simple,specific and rapid LC-MS/MS method was established to determine flumatinib and its two major metabolites in human plasma for clinical therapeutic drug monitoring.Methods The determination was performed on an ACQUITY UPLC HSS T3 column(2.1 mm×50 mm,1.8 μm)with mobile phases consisting of acetonitrile and 10 mmol·L-1 ammonium formate(containing 0.1%formic acid)with gradient elution at the flow rate of 0.5 mL·min-1.The elution time was 6 min.The temperature of the column was 38℃.The ion source was electrospray ion source and the scanning mode was multiple reaction monitoring scanning in positive ion mode.Results The mass concentrations of flumatinib and its metabolites(flumatinib M1 and flumatinib M3)have a good linear relationship within the concentration range investigated.The precision and stability of the method are good.The precision is less than 15%,and the relative deviation is within±15%.The extraction recoveries of flumatinib and its metabolites approach nearly 100%.Conclusion The method is simple and sensitive,and can accurately determine the plasma concentration of flumatinib and its metabolites,providing a basis for clinical rational drug use.关键词
氟马替尼/液相色谱串联质谱/血药浓度Key words
Flumatinib/Liquid chromatography tandem mass spectrometry/Plasma concentration分类
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徐月华,钱洲逸,赵杨,黄琼叶,孙鲁宁,王永庆,孙志明,唐雯雯..LC-MS/MS法同时测定血浆中氟马替尼及其代谢产物浓度[J].医药导报,2023,42(12):1779-1784,6.
