医药导报2023，Vol.42Issue(12)：1796-1801,6.DOI:10.3870/j.issn.1004-0781.2023.12.009
高效液相色谱-串联质谱法同时测定大鼠血浆中7种B族维生素的含量
Simultaneous Determination of Seven B Vitamins in Rat Plasma by High-performance Liquid Chromatography-tandem Mass Spectrometry
摘要
Abstract
Objective To establish a high-performance liquid chromatography-tandem mass spectrometry(LC-MS/MS)method for the simultaneous determination of seven vitamin Bs(VBs)in rat plasma.Methods The plasma samples were pretreated using the protein precipitation method.The chromatographic separation was achieved using an Agilent ZORBAX SB-Aq column(2.1 mm×150 mm,3.5 μm),with a mobile phase composed of 0.01%formic acid(A)and methanol(B)in a gradient elution mode.The flow rate was set at 0.3 mL·min-1 and the column temperature was maintained at 30℃.Mass spectrometric detection was performed using positive ion mode in multiple reaction monitoring.2,4,5,6-Deuteronicotinamide was used as the internal standard.Results Methodological validation results showed that the lowest limits of quantitation(LOQs)for the seven VBs ranged from 7.5 to 300 ng/mL,while the highest LOQs ranged from 1 000 to 20 000 ng·mL-1.Within a certain concentration range,all compounds showed a good linearity(r2>0.992 2).The accuracies ranged from 86.0%to 117.6%,and both intra-and inter-batch precision were less than 20%.Additionally,all analytes demonstrated extraction recoveries greater than 86.1%.No significant matrix effects were observed,and the stability was good.The established validation method was successfully applied to the pharmacokinetics study of single intragastric administration of VB solution in SD rats.Conclusion The analytical method established in this study is simple,rapid,sensitive,and exclusive for the simultaneous determination of VB in real plasma samples.This LC-MS/MS method provides a new analytical tool for further exploring the physiological and pathological effects of VB.关键词
B族维生素/高效液相色谱-串联质谱法/定量分析Key words
Vitamin Bs/High-performance liquid chromatography-tandem mass spectrometry/Quantitative analysis/Method validation分类
药学引用本文复制引用
余霞霞,曾语桐,杨佳宏,吴婧,徐燕,黄寅..高效液相色谱-串联质谱法同时测定大鼠血浆中7种B族维生素的含量[J].医药导报,2023,42(12):1796-1801,6.基金项目
江苏省药学会-天晴(连云港)医院药学科研项目(Q202013) （连云港）
中国药科大学大学生创新创业训练计划项目(2023055). （2023055）
