湖北医药学院学报2023，Vol.42Issue(6)：579-583,封2,6.DOI:10.13819/j.issn.2096-708X.2023.06.001
虎杖粗提物对宫颈癌Hela细胞增殖与迁移作用机制研究
Mechanism Study on the Effects of Polygonum Cuspidatum Extract on Proliferation and Migration of Cervical Cancer Hela Cells
摘要
Abstract
Objective To investigate the mechanism of Polygonum cuspidatum extract on proliferation and migration of cervi-cal cancer Hela cells.Methods Cell Counting Kit-8(CCK-8),adenosine 5'-triphosphate(ATP)and lactate dehydro-genase(LDH)assay kits were used to evaluate the influence of Polygonum cuspidatum extract on the proliferation of Hela cells.The scratch assay was performed to assess the effect of Polygonum cuspidatum extract on the horizontal migration abil-ity of Hela cells.Flow cytometry was employed to detect the impact of Polygonum cuspidatum extract on apoptosis of Hela cells.Western blotting was conducted to analyze the expression levels of caspase 1/3/8/9,poly ADP-ribose polymerase(PARP)and Gasdermin E(GSDME)proteins in Hela cells treated with Polygonum cuspidatum extract.Results The crude extract of Polygonum cuspidatum inhibited the proliferation of Hela cells,significantly inhibited their migration abili-ty,induced Hela cell apoptosis,and significantly increased the expression levels of apoptosis related proteins such as cleaved caspase3/8/9 and PARP in Hela cells.Conclusion Polygonum cuspidatum extract can effectively inhibit the prolif-eration and migration of Hela cells,possibly through inducing apoptosis and causing mitochondrial dysfunction.关键词
虎杖粗提物/宫颈癌Hela细胞/凋亡/线粒体功能障碍Key words
Polygonum cuspidatum extract/Cervical cancer Hela cells/Apoptosis/Mitochondrial dysfunction引用本文复制引用
李彤晖,杨佳慧,李琛..虎杖粗提物对宫颈癌Hela细胞增殖与迁移作用机制研究[J].湖北医药学院学报,2023,42(6):579-583,封2,6.基金项目
中央本级重大增减支项目(2060302) （2060302）
