湖北医药学院学报2023，Vol.42Issue(6)：590-593,4.DOI:10.13819/j.issn.2096-708X.2023.06.003
缺氧微环境对U2OS骨肉瘤细胞化疗耐药性的影响
Effect of Hypoxic Microenvironment on Chemotherapy Resistance in U2OS Osteosarcoma Cells
摘要
Abstract
Objective To detect the effect of anti-tumor drugs in osteosarcoma U2OS cells under low oxygen concentration,so as to explore the impact of hypoxic microenvironment on the chemotherapy resistance of osteosarcoma U2OS cells.Meth-ods U2OS osteosarcoma cells were cultured through in vitro experiments,and cells were treated with different concentra-tions of low oxygen inducer CoCl2 for 24 hours.The proliferation of cells in each group was detected by CCK-8,and the op-timal concentration of CoCl2 intervention was established.After adding different concentrations of cisplatin,the proliferation of osteosarcoma cells in each group was detected using CCK-8,and the proportion of cell apoptosis in each group was de-tected by flow cytometry.Results When osteosarcoma U2OS cells were in hypoxic environment,their proliferation ability was enhanced,and there was a significant difference compared with the control group(P＜0.05).CCK-8 results showed that when the cells were in hypoxic environment,antitumor drugs could inhibit the proliferation of osteosarcoma U2OS cells in a dose-dependent manner.Flow cytometry results showed that the apoptosis rate of osteosarcoma U2OS cells after cispla-tin treatment was positively correlated with the drug concentration(P＜0.05).Conclusion Osteosarcoma U2OS cells have stronger proliferative ability under hypoxic conditions and antagonize antitumor drug-mediated the proliferation inhibition and apoptotic promotion,thereby enhancing chemotherapy resistance.关键词
骨肉瘤/缺氧微环境/抗肿瘤药物/化疗耐药性Key words
Osteosarcoma/Hypoxic microenvironment/Antineoplastic drugs/Chemotherapy resistance引用本文复制引用
黄成校,余化龙,高超,冯浩,武伦..缺氧微环境对U2OS骨肉瘤细胞化疗耐药性的影响[J].湖北医药学院学报,2023,42(6):590-593,4.基金项目
十堰市科技局引导性项目(21Y88) （21Y88）
