针刺研究2023，Vol.48Issue(12)：1202-1208,1235,8.DOI:10.13702/j.1000-0607.20220877
艾灸通过白细胞介素-33/生长刺激表达基因2蛋白通路影响阿尔茨海默病小鼠海马小胶质细胞极化
Moxibustion influences hippocampal microglia polarization via IL-33/ST2 pathway in Alzheimer's disease mice
摘要
Abstract
Objective To observe the effect of moxibustion on the polarization of microglia towards M2 direc-tion in Alzheimer's disease(AD)mice through the interleukin-33(IL-33)/growth stimulating gene 2 protein(ST2)sig-naling pathway.Methods Five-month-old APP/PS1 male mice were randomly divided into model and moxibustion(Moxi)groups,and C57BL/6J mice of the same age were as the control group,with 9 mice in each group.In the Moxi group,moxibustion was applied at"Baihui"(GV20)and"Yongquan"(KI1)for 30 min,once a day,5 days a week for 4 weeks.The spatial learning memory ability was observed by the Morris water maze test.The relative expressions of IL-33 and ST2 in hippocampus were detected by Western blot.The positive expression of amyloid-β(Aβ),phosphorylated Tau(p-Tau),IL-33/ionized calcium binding adapter molecule 1(Iba-1),ST2/Iba-1,arginase 1(Arg1)/Iba-1 and indu-cible nitric oxide synthase(iNOS)/Iba-1 in hippocampal CA1 region were detected by immunofluorescence.Results Compared with the control group,the escape latency of the mice in the model group was prolonged(P<0.001,P<0.01),the number of times to enter the effective area and the percentage of target quadrant swimming time were re-duced(P<0.001),the positive expression of both Aβ and p-Tau,the positive expression of iNOS/Iba-1 in the hippocam-pal CA1 region was increased(P<0.001),while the expression of IL-33 and ST2 protein in hippocampal tissue,the positive expression levels of IL-33/Iba-1,ST2/Iba-1 and Arg1/Iba-1 in hippocampal CA1 region were all decreased(P<0.05,P<0.001).After treatment,compared with the model group,the escape latency of the mice in the moxibustion group was shortened(P<0.001,P<0.01),the number of entries into the effective area and the percentage of target quadrant swimming time were increased(P<0.001),the positive expression of Aβ and p-Tau in the hippocampal CA1 region,and the positive expression of iNOS/Iba-1 were decreased(P<0.001),while the expression of IL-33 and ST2 protein in the hippocampal tissue,the positive expression of IL-33/Iba-1,ST2/Iba-1 and Arg1/Iba-1 in hippocampal CA1 region were all increased(P<0.05,P<0.01,P<0.001).Conclusion Moxibustion can improve the spatial learning and memory abilities,reduce the pathological deposition of Aβ and p-Tau in APP/PS1 mice,which may be related to its function in up-regulating the IL-33/ST2 signaling pathway to regulate the polarization of microglia towards M2 direction.关键词
艾灸/阿尔茨海默病/IL-33/ST2信号通路/小胶质细胞极化Key words
Moxibustion/Alzheimer's disease/IL-33/ST2 signaling pathway/Microglia polarization分类
医药卫生引用本文复制引用
李虹莹,沈缘,谢璐霜,吴巧凤,余曙光..艾灸通过白细胞介素-33/生长刺激表达基因2蛋白通路影响阿尔茨海默病小鼠海马小胶质细胞极化[J].针刺研究,2023,48(12):1202-1208,1235,8.基金项目
国家自然科学基金项目(No.81873386、82174511、82174512) （No.81873386、82174511、82174512）
