针刺研究2023，Vol.48Issue(12)：1242-1248,7.DOI:10.13702/j.1000-0607.20221305
电针对帕金森病小鼠铁死亡诱导的氧化应激及细胞凋亡的影响
Effect of electroacupuncture on oxidative stress and cell apoptosis induced by ferroptosis in mice with Parkinson's disease
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)on ferroptosis and apoptosis-related proteins in the substantia nigra of midbrain in mice with Parkinson's disease(PD),so as to explore its possible mecha-nisms in the treatment of PD.Methods Twenty-four C57BL/6 mice were randomly divided into blank,model and EA groups,with 8 mice in each group.The PD model was established by continuous gavage of rotenone for 4 weeks.EA was applied at"Baihui"(GV20),"Quchi"(LI11)and"Zusanli"(ST36)for 20 min,once a day for 14 days,with 2-day rest after every 5-day treatment.The open field test was used to evaluate the residence time in the central area,ave-rage movement speed,and total distance of the open field.Western blot was used to detect the protein expression le-vels of divalent metal ion transporter 1(DMT1),membrane ferroportin 1(FPN1),glutathione peroxidase 4(GPX4),proapoptotic protein Bax,and anti apoptotic protein Bcl-2 in the substantia nigra.Immunohistochemical method was used to detect the morphological changes of neurons and the positive expression of tyrosine hydroxylase(TH)in the substantia nigra of mice.Results After 4 weeks of modeling,compared with the blank group,the residence time in the central area,average speed and total distance of open field were significantly lower(P<0.0001,P<0.01,P<0.001);the protein expression levels of DMT1 and Bax in the substantia nigra were increased(P<0.001,P<0.0001),while the protein expression levels of FPN1,GPX4 and Bcl-2,and the optical density of TH+ cells in the substantia nigra were de-creased(P<0.0001,P<0.001)in the model group.In comparison with the model group,the residence time in the cen-tral area,average speed,and total distance of the EA group were increased(P<0.01,P<0.05);the protein expression levels of DMT1 and Bax in the substantia nigra were decreased(P<0.01,P<0.001),while the protein expression levels of FPN1,GPX4,and Bcl-2,and the optical density of TH+ cells in the substantia nigra were increased(P<0.0001,P<0.01,P<0.001,P<0.05).Conclusion EA has a protective effect on dopaminergic neurons in the substantia nigra of midbrain in PD model mice,which may be related with its effect in regulating oxidative stress and cell apoptosis induced by ferroptosis.关键词
帕金森病/电针/铁死亡/氧化应激/细胞凋亡Key words
Parkinson's disease/Electroacupuncture/Ferroptosis/Oxidative stress/Cell apoptosis分类
医药卫生引用本文复制引用
马雪,刘凡,王强,王渊,刘智斌..电针对帕金森病小鼠铁死亡诱导的氧化应激及细胞凋亡的影响[J].针刺研究,2023,48(12):1242-1248,7.基金项目
国家自然科学基金项目(No.82204904) （No.82204904）
陕西中医药大学博士科研基金项目(No.306-17102032232) （No.306-17102032232）
陕西省教育厅科学研究计划(No.22JS007) （No.22JS007）
陕西省"双链融合"中青年科研创新团队项目(No.2022-SLRH-LJ-012) （No.2022-SLRH-LJ-012）
