针刺研究2023，Vol.48Issue(12)：1249-1257,9.DOI:10.13702/j.1000-0607.20220841
电针"足三里"减轻大肠癌小鼠化疗后肠黏膜损伤及其对氧化应激和细胞凋亡的影响
Protective effect of electroacupuncture at ST36 against damage of intestinal mucosa,oxidative stress and apoptosis induced by 5-FU chemotherapy in mice with colon cancer
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)at"Zusanli"(ST36)on intestinal mu-cosal damage,intestinal mucosal oxidative stress injury and apoptosis induced by 5-fluorouraeil(5-FU)chemotherapy in colorectal cancer-bearing mice.Methods Thirty male BALB/c mice were randomly divided into normal control,colorectal cancer(CT26),5-FU,non-acupoint and ST36 groups,with 6 mice in each group.Except for those of the nor-mal control group,mice of the remaining 4 groups received subcutaneous implantation of colorectal CT26 cell suspen-sion(0.1 mL)in the right armpit for establishing colorectal cancer model.Rats of the 5-FU group,non-acupoint group and ST36 group were given with 5 mg/mL 5-FU solution once every 3 days for a total of 21 days.For mice of the non-acupoint group and ST36 group,EA(2 Hz,1-2 mA)was applied to bilateral ST36 or non-acupoints(the bilateral sunken spots about 3 mm to the midpoint between the tail root and the anus)for 5 min after each intraperitoneal infu-sion of 5-FU,once every 3 days,for a total of 21 days.After the intervention,the diarrhea index was assessed.The length of colon(from the endpoint of cecum to the anal orifice)was measured.Histopathological changes of colonic mu-cosa were observed by H.E.staining,and the length of colonic villi was measured.The content of malondialdehyde(MDA),and activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)of colonic tissue were de-tected by thibabituric acid,xanthine oxidase and colorimetric method,respectively.The rate of cell apoptosis in the co-lonic tissue was measured by TUNEL assay.The positive expressions of Bax and Bcl-2 in colonic tissue were deter-mined by immunohistochemistry.Results The CT26 model group didn't show any significant changes in the diarrhea index,colon length,colon villus length,MDA content,SOD and GSH-Px activities,colonic cell apoptosis rate,and Bax and Bcl-2 expression levels when compared with the normal group.Compared with the CT26 group,the 5-FU group had a remarkable increase in the diarrhea index,MDA content,colonic cell apoptosis rate and Bax expression level(P<0.01,P<0.05),and a marked decrease in the colon length,colon villus length,SOD and GSH-Px activities and Bcl-2 expression level(P<0.01),suggesting the side effects of administration of 5-FU.Compared with the 5-FU group,the diarrhea index,MDA content,colonic cell apoptosis rate and Bax expression level were markedly decreased(P<0.05,P<0.01)and those of the colon length,colon villus length,SOD and GSH-Px activities and Bcl-2 expression level were obviously increased(P<0.01)in the ST36 group.Compared with the 5-FU group,the non-acupoint group also had an increase in the colon villus length,SOD and GSH-Px activities(P<0.01,P<0.05)and a decrease in the cell apoptosis rate(P<0.01).Conclusion EA at ST36 has a positive effect in reducing intestinal mucosal damage induced by 5-FU chemotherapy in cancer-bearing mice,which may be related to its function in relieving oxidative stress injury and inhibiting apoptosis of colonic tissue.关键词
电针/5-氟尿嘧啶化疗/肠黏膜损伤/足三里/氧化应激/细胞凋亡Key words
Electroacupuncture/5-FU chemotherapy/Damage of intestinal mucosa/Zusanli(ST36)/Oxidative stress/Apoptosis分类
医药卫生引用本文复制引用
张学君,林久茂,林晨捷,彭娇,杨晓丹,陈诗兰,赵锦燕..电针"足三里"减轻大肠癌小鼠化疗后肠黏膜损伤及其对氧化应激和细胞凋亡的影响[J].针刺研究,2023,48(12):1249-1257,9.基金项目
福建中医药大学针灸学科开放课题(No.XZJ2022001) （No.XZJ2022001）
