中国病理生理杂志2023，Vol.39Issue(12)：2133-2140,8.DOI:10.3969/j.issn.1000-4718.2023.12.003
飞龙掌血通过调控大鼠M1/M2型小胶质细胞极化减轻脑缺血再灌注损伤
Toddalia asiatica attnuates cerebral ischemia-reperfusion injury in rats through modulating M1/M2 polarization of microglia
摘要
Abstract
AIM:Observation of neuroprotective effects of Toddalia asiatica(TA)on cerebral ischemia-reper-fusion injury(CIRI)in rats by investigating the effects and mechanisms of drugs on the polarization of microglia M1/M2 subtype and the Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear factor-κB(NF-κB)path-way.METHODS:The modified thread occlusion method was used to establish a rat model of CIRI,and the rats were ran-domly divided into the model group,Toddalia asiatica(1.08 g/kg)group,donepezil hydrochloride(0.45 mg/kg)group,and sham group,with 16 rats in each group.Based on the assessment of neurofunctional changes in each group of rats,HE and Nissl staining were used to observe the pathological changes in brain tissue.TUNEL staining was performed to de-tect neuronal apoptosis,immunohistochemistry was used to detect the expression of M1 microglia marker ionized calcium-binding adapter molecule 1(Iba1),M2 microglia marker arginase 1(Arg1),and TLR4 localization.Western blot was used to detect the expression of microglia polarization proteins and proteins related to TLR4/MyD88/NF-κB pathway in the hippocampus region.RESULTS:Compared with sham group,the model group rats had higher neurological function scores(P＜0.01),and neuronal arrangement in the hippocampus and cortex was loose and disordered,Nissl bodies de-creased,and neuronal apoptosis increased.The numbers of M1 microglia marker Iba1-,M2 microglia marker Arg1-,and TLR4-positive cells were significantly increased.In addition,the protein levels of TLR4,MyD88,p-NF-κB p65,NF-κB p65,p-NF-κB inhibitory factor(p-IκB),Iba1,interleukin-6(IL-6),and Arg1 in the hippocampus were elevated(P＜0.05),while IL-4 and IL-10 expression were decreased(P＜0.01).Compared with model group,the Toddalia asiatica group and Donepezil hydrochloride group showed increased protein expression of Arg1,IL-4 and IL-10(P＜0.05),while the other indicators were decreased(P＜0.05,P＜0.01).CONCLUSION:Toddalia asiatica possesses neuroprotective effects on CIRI rats,which may be attributed to its ability to regulate M1/M2 polarization and inhibit the TLR4/MyD88/NF-κB-mediated inflammatory pathway.关键词
飞龙掌血/脑缺血再灌注损伤/小胶质细胞/神经炎症/凋亡Key words
Toddalia asiatica/cerebral ischemia-reperfusion injury/microglia/neuroinflammation/apopto-sis分类
医药卫生引用本文复制引用
高建红,王刚,杨丹,赵方毓,何一多,陈显兵..飞龙掌血通过调控大鼠M1/M2型小胶质细胞极化减轻脑缺血再灌注损伤[J].中国病理生理杂志,2023,39(12):2133-2140,8.基金项目
国家自然科学基金资助项目(No.82260821) （No.82260821）
恩施州科技局项目(No.JCY2021000054 （）
No.2018-15) （）
湖北民族大学附属民大医院开放课题项目(No.OIR202301Q) （No.OIR202301Q）
湖北民族大学研究生教育创新项目(No.MYK2023069) （No.MYK2023069）
